Using redox-sensitive fluorescent probes to record real-time reactive oxygen species production in cells from mouse carotid body slices

Abstract

Reactive oxygen species (ROS) are important signaling molecules for physiologic processes such as acute response to hypoxia. However, reliable real-time ROS measurement in cells has been a long-standing methodological challenge. Here, we present a protocol to record acute changes in ROS production in sen- sory cells from mouse carotid body (CB) slices using redox-sensitive green fluo- rescent protein probes and microfluorimetry. This protocol provides sensitive and reproducible quantification of ROS during acute hypoxia in different subcel- lular compartments of CB glomus cells.