Artículo
Using redox-sensitive fluorescent probes to record real-time reactive oxygen species production in cells from mouse carotid body slices
Autor/es | Gao Chen, Lin
Arias-Mayenco, Ignacio Ortega Sáenz, Patricia López Barneo, José |
Departamento | Universidad de Sevilla. Departamento de Fisiología Médica y Biofisica |
Fecha de publicación | 2021 |
Fecha de depósito | 2022-10-25 |
Publicado en |
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Resumen | Reactive oxygen species (ROS) are important signaling molecules for physiologic
processes such as acute response to hypoxia. However, reliable real-time ROS
measurement in cells has been a long-standing methodological ... Reactive oxygen species (ROS) are important signaling molecules for physiologic processes such as acute response to hypoxia. However, reliable real-time ROS measurement in cells has been a long-standing methodological challenge. Here, we present a protocol to record acute changes in ROS production in sen- sory cells from mouse carotid body (CB) slices using redox-sensitive green fluo- rescent protein probes and microfluorimetry. This protocol provides sensitive and reproducible quantification of ROS during acute hypoxia in different subcel- lular compartments of CB glomus cells. |
Agencias financiadoras | Ministerios de Ciencia e Innovación y Sanidad de España |
Identificador del proyecto | SAF2012-39343
AF2016-74990-R |
Cita | Gao Chen, L., Arias-Mayenco, I., Ortega Sáenz, P. y López Barneo, J. (2021). Using redox-sensitive fluorescent probes to record real-time reactive oxygen species production in cells from mouse carotid body slices. STAR Protocols, 2 (2), 100535. https://doi.org/10.1016/j.xpro.2021.100535. |
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