Artículo
PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ
Autor/es | Casado Combreras, Miguel Ángel
Rivero Rodríguez, Francisco Elena-Real, Carlos A. Molodenskiy, Dmitry Díaz Quintana, Antonio Jesús Martinho, Marlène Gerbaud, Guillaume González Arzola, Katiuska Velázquez Campoy, Adrián Svergun, Dmitri Belle, Valérie Rosa Acosta, Miguel Ángel de la Díaz Moreno, Irene |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-09-01 |
Publicado en |
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Resumen | Intrinsic protein flexibility is of overwhelming relevance for intermolecular recognition and adaptability of highly dynamic ensemble of complexes, and the phenomenon is essential for the understanding of numerous biological ... Intrinsic protein flexibility is of overwhelming relevance for intermolecular recognition and adaptability of highly dynamic ensemble of complexes, and the phenomenon is essential for the understanding of numerous biological processes. These conformational ensembles—encounter complexes—lack a unique organization, which prevents the determination of well-defined high resolution structures. This is the case for complexes involving the oncoprotein SET/template-activating factor-Iβ (SET/TAF-Iβ), a histone chaperone whose functions and interactions are significantly affected by its intrinsic structural plasticity. Besides its role in chromatin remodeling, SET/TAF-Iβ is an inhibitor of protein phosphatase 2A (PP2A), which is a key phosphatase counteracting transcription and signaling events controlling the activity of DNA damage response (DDR) mediators. During DDR, SET/TAF-Iβ is sequestered by cytochrome c (Cc) upon migration of the hemeprotein from mitochondria to the cell nucleus. Here, we report that the nuclear SET/TAF-Iβ:Cc polyconformational ensemble is able to activate PP2A. In particular, the N-end folded, globular region of SET/TAF-Iβ (a.k.a. SET/TAF-Iβ ΔC)—which exhibits an unexpected, intrinsically highly dynamic behavior—is sufficient to be recognized by Cc in a diffuse encounter manner. Cc-mediated blocking of PP2A inhibition is deciphered using an integrated structural and computational approach, combining small-angle X-ray scattering, electron paramagnetic resonance, nuclear magnetic resonance, calorimetry and molecular dynamics simulations. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | PID2021-126663NB-I00
PGC2018-096049-B-I00 BFU2015- 71017 BIO-198, US-1254317 US-1257019 P18-FR3487 P18-HO-4091 |
Cita | Casado Combreras, M.Á., Rivero Rodríguez, F., Elena-Real, C.A., Molodenskiy, D., Díaz Quintana, A.J., Martinho, M.,...,Díaz Moreno, I. (2022). PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ. Computational and Structural Biotechnology Journal, 20, 3695-3707. |
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