dc.creator | San Martín Alonso, Marta | es |
dc.creator | Soler Oliva, María Eugenia | es |
dc.creator | García Rubio, María Luisa | es |
dc.creator | García Muse, Tatiana | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2021-08-31T15:20:02Z | |
dc.date.available | 2021-08-31T15:20:02Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | San Martín Alonso, M., Soler Oliva, M.E., García Rubio, M.L., García Muse, T. y Aguilera López, A. (2021). Harmful R-loops are prevented via different cell cycle-specific mechanisms. Nature Communications, 12 (1), 4451. | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | https://hdl.handle.net/11441/125249 | |
dc.description.abstract | Identifying how R-loops are generated is crucial to know how transcription compromises genome integrity. We show by genome-wide analysis of conditional yeast mutants that the THO transcription complex, prevents R-loop formation in G1 and S-phase, whereas the Sen1 DNA-RNA helicase prevents them only in S-phase. Interestingly, damage accumulates asymmetrically downstream of the replication fork in sen1 cells but symmetrically in the hpr1 THO mutant. Our results indicate that: R-loops form co-transcriptionally independently of DNA replication; that THO is a general and cell-cycle independent safeguard against R-loops, and that Sen1, in contrast to previously believed, is an S-phase-specific R-loop resolvase. These conclusions have important implications for the mechanism of R-loop formation and the role of other factors reported to affect on R-loop homeostasis. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2016-75058-P | es |
dc.description.sponsorship | European Research Council ERC2014 AdG669898 TARLOOP | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | Springer Nature | es |
dc.relation.ispartof | Nature Communications, 12 (1), 4451. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Harmful R-loops are prevented via different cell cycle-specific mechanisms | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BFU2016-75058-P | es |
dc.relation.projectID | ERC2014 AdG669898 TARLOOP | es |
dc.relation.publisherversion | https://doi.org/10.1038/s41467-021-24737-x | es |
dc.identifier.doi | 10.1038/s41467-021-24737-x | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 4451 | es |