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dc.creatorOrtega Moreno, Pedroes
dc.creatorMérida Cerro, José Antonioes
dc.creatorGarcía Rondón, Anaes
dc.creatorGómez González, Belénes
dc.creatorAguilera López, Andréses
dc.date.accessioned2021-08-31T15:06:43Z
dc.date.available2021-08-31T15:06:43Z
dc.date.issued2021
dc.identifier.citationOrtega Moreno, P., Mérida Cerro, J.A., García Rondón, A., Gómez González, B. y Aguilera López, A. (2021). Dna-rna hybrids at dsbs interfere with repair by homologous recombination. eLife, 10, e69881.
dc.identifier.issn2050-084Xes
dc.identifier.urihttps://hdl.handle.net/11441/125248
dc.description.abstractDNA double-strand breaks (DSBs) are the most harmful DNA lesions and their repair is crucial for cell viability and genome integrity. The readout of DSB repair may depend on whether DSBs occur at transcribed versus non-transcribed regions. Some studies have postulated that DNA-RNA hybrids form at DSBs to promote recombinational repair, but others have challenged this notion. To directly assess whether hybrids formed at DSBs promote or interfere with the recombinational repair, we have used plasmid and chromosomal-based systems for the analysis of DSB-induced recombination in Saccharomyces cerevisiae. We show that, as expected, DNA-RNA hybrid formation is stimulated at DSBs. In addition, mutations that promote DNA-RNA hybrid accumulation, such as hpr1∆ and rnh1∆ rnh201∆, cause high levels of plasmid loss when DNA breaks are induced at sites that are transcribed. Importantly, we show that high levels or unresolved DNA-RNA hybrids at the breaks interfere with their repair by homologous recombination. This interference is observed for both plasmid and chromosomal recombination and is independent of whether the DSB is generated by endonucleolytic cleavage or by DNA replication. These data support a model in which DNA-RNA hybrids form fortuitously at DNA breaks during transcription and need to be removed to allow recombinational repair, rather than playing a positive role.es
dc.description.sponsorshipEuropean Research Council ERC2014 AdG669898 TARLOOPes
dc.description.sponsorshipMinisterio de Economía y Competitividad BFU2016-75058-Pes
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades PDI2019-104270GB-I00es
dc.formatapplication/pdfes
dc.format.extent22 p.es
dc.language.isoenges
dc.publishereLife Sciences Publicationses
dc.relation.ispartofeLife, 10, e69881.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDna-rna hybrids at dsbs interfere with repair by homologous recombinationes
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDERC2014 AdG669898 TARLOOPes
dc.relation.projectIDBFU2016-75058-Pes
dc.relation.projectIDPDI2019-104270GB-I00es
dc.relation.publisherversionhttps://doi.org/10.7554/eLife.69881es
dc.identifier.doi10.7554/eLife.69881es
dc.journaltitleeLifees
dc.publication.volumen10es
dc.publication.initialPagee69881es

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