Repositorio de producción científica de la Universidad de Sevilla

Molecular basis of FIR-mediated c-myc transcriptional control

 

Advanced Search
 
Opened Access Molecular basis of FIR-mediated c-myc transcriptional control
Cites

Show item statistics
Icon
Export to
Author: Cukier, Cyprian D.
Hollingworth, David
Martin, Stephen
Kelly, Geoff
Díaz Moreno, Irene
Ramos, Andrés
Department: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Date: 2010
Published in: Nature Structural and Molecular Biology, 17 (9), 1058-1064.
Document type: Article
Abstract: The far upstream element (FUSE) regulatory system promotes a peak in the concentration of c-Myc during cell cycle. First, the FBP transcriptional activator binds to the FUSE DNA element upstream of the c-myc promoter. Then, FBP recruits its specific repressor (FIR), which acts as an on/off transcriptional switch. Here we describe the molecular basis of FIR recruitment, showing that the tandem RNA recognition motifs of FIR provide a platform for independent FUSE DNA and FBP protein binding and explaining the structural basis of the reversibility of the FBP-FIR interaction. We also show that the physical coupling between FBP and FIR is modulated by a flexible linker positioned sequentially to the recruiting element. Our data explain how the FUSE system precisely regulates c-myc transcription and suggest that a small change in FBP-FIR affinity leads to a substantial effect on c-Myc concentration.
Cite: Cukier, C.D., Hollingworth, D., Martin, S., Kelly, G., Díaz Moreno, I. y Ramos, A. (2010). Molecular basis of FIR-mediated c-myc transcriptional control. Nature Structural and Molecular Biology, 17 (9), 1058-1064.
Size: 1.574Mb
Format: PDF

URI: https://hdl.handle.net/11441/84706

DOI: 10.1038/nsmb.1883

See editor´s version

This work is under a Creative Commons License: 
Attribution-NonCommercial-NoDerivatives 4.0 Internacional

This item appears in the following Collection(s)