Artículo
Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella
Autor/es | Papenfort, Kai
Espinosa Alfaro, Elena Casadesús Pursals, Josep Vogelb, Jörg |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2015 |
Fecha de depósito | 2018-01-17 |
Publicado en |
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Resumen | Horizontal gene transfer via plasmid conjugation is a major driving force in microbial evolution but constitutes a complex process that requires synchronization with the physiological state of the host bacteria. Although ... Horizontal gene transfer via plasmid conjugation is a major driving force in microbial evolution but constitutes a complex process that requires synchronization with the physiological state of the host bacteria. Although several host transcription factors are known to regulate plasmid-borne transfer genes, RNA-based regulatory circuits for host-plasmid communication remain unknown. We describe a posttranscriptional mechanism whereby the Hfq-dependent small RNA, RprA, inhibits transfer of pSLT, the virulence plasmid of Salmonella enterica. RprA employs two separate seed-pairing domains to activate the mRNAs of both the sigma-factor σS and the RicI protein, a previously uncharacterized membrane protein here shown to inhibit conjugation. Transcription of ricI requires σS and, together, RprA and σS orchestrate a coherent feedforward loop with AND-gate logic to tightly control the activation of RicI synthesis. RicI interacts with the conjugation apparatus protein TraV and limits plasmid transfer under membrane-damaging conditions. To our knowledge, this study reports the first small RNA-controlled feedforward loop relying on posttranscriptional activation of two independent targets and an unexpected role of the conserved RprA small RNA in controlling extrachromosomal DNA transfer. |
Identificador del proyecto | BIO2013-44220-R
CSD2008-00013 CVI-5879 |
Cita | Papenfort, K., Espinosa Alfaro, E., Casadesús Pursals, J. y Vogelb, J. (2015). Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella. Proceedings of the National Academy of Sciences of the United States of America, 112 (34), E4772-E4781. |
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