dc.creator | Papenfort, Kai | es |
dc.creator | Espinosa Alfaro, Elena | es |
dc.creator | Casadesús Pursals, Josep | es |
dc.creator | Vogelb, Jörg | es |
dc.date.accessioned | 2018-01-17T16:01:32Z | |
dc.date.available | 2018-01-17T16:01:32Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Papenfort, K., Espinosa Alfaro, E., Casadesús Pursals, J. y Vogelb, J. (2015). Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella. Proceedings of the National Academy of Sciences of the United States of America, 112 (34), E4772-E4781. | |
dc.identifier.issn | 0027-8424 (impreso) | es |
dc.identifier.issn | 1091-6490 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/69133 | |
dc.description.abstract | Horizontal gene transfer via plasmid conjugation is a major driving force in microbial evolution but constitutes a complex process that requires synchronization with the physiological state of the host bacteria. Although several host transcription factors are known to regulate plasmid-borne transfer genes, RNA-based regulatory circuits for host-plasmid communication remain unknown. We describe a posttranscriptional mechanism whereby the Hfq-dependent small RNA, RprA, inhibits transfer of pSLT, the virulence plasmid of Salmonella enterica. RprA employs two separate seed-pairing domains to activate the mRNAs of both the sigma-factor σS and the RicI protein, a previously uncharacterized membrane protein here shown to inhibit conjugation. Transcription of ricI requires σS and, together, RprA and σS orchestrate a coherent feedforward loop with AND-gate logic to tightly control the activation of RicI synthesis. RicI interacts with the conjugation apparatus protein TraV and limits plasmid transfer under membrane-damaging conditions. To our knowledge, this study reports the first small RNA-controlled feedforward loop relying on posttranscriptional activation of two independent targets and an unexpected role of the conserved RprA small RNA in controlling extrachromosomal DNA transfer. | es |
dc.description.sponsorship | Bildung und Forschung Project eBio:RNAsys BIO2013-44220-R | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad CSD2008-00013 | es |
dc.description.sponsorship | Junta de Andalucía CVI-5879 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | National Academy of Sciences | es |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America, 112 (34), E4772-E4781. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Feedforward control | es |
dc.subject | Hfq | es |
dc.subject | Plasmid conjugation | es |
dc.subject | RprA | es |
dc.subject | SRNA | es |
dc.title | Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BIO2013-44220-R | es |
dc.relation.projectID | CSD2008-00013 | es |
dc.relation.projectID | CVI-5879 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1073/pnas.1507825112 | es |
dc.identifier.doi | 10.1073/pnas.1507825112 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Proceedings of the National Academy of Sciences of the United States of America | es |
dc.publication.volumen | 112 | es |
dc.publication.issue | 34 | es |
dc.publication.initialPage | E4772 | es |
dc.publication.endPage | E4781 | es |