Repositorio de producción científica de la Universidad de Sevilla

The presence of glutamate dehydrogenase is a selective advantage for the cyanobacterium Synechocystis sp. strain PCC 6803 under nonexponential growth conditions

Opened Access The presence of glutamate dehydrogenase is a selective advantage for the cyanobacterium Synechocystis sp. strain PCC 6803 under nonexponential growth conditions
Estadísticas
Icon
Exportar a
Autor: Chávez de Diego, Sebastián
Lucena Soto, José Manuel
Reyes Rosa, José Carlos
Florencio Bellido, Francisco Javier
Candau Chacón, Pedro
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 1999
Publicado en: Journal of Bacteriology, 181 (3), 808-813.
Tipo de documento: Artículo
Resumen: he unicellular cyanobacterium Synechocystis sp. strain PCC 6803 has two putative pathways for ammonium assimilation: the glutamine synthetase- glutamate synthase cycle, which is the main one and is finely regulated by the nitrogen source; and a high NADP-dependent glutamate dehydrogenase activity (NADP-GDH) whose contribution to glutamate synthesis is uncertain. To investigate the role of the latter, we used two engineered mutants, one lacking and another overproducing NADP-GDH. No major disturbances in the regulation of nitrogen-assimilating enzymes or in amino acids pools were detected in the null mutant, but phycobiline content, a sensitive indicator of the nutritional state of cyanobacterial cells, was significantly reduced, indicating that NADP-GDH plays an auxiliary role in ammonium assimilation. This effect was already prominent in the initial phase of growth, although differences in growth rate between the wild type and the mutants were observed at this stage only at low light...
[Ver más]
Cita: Chávez de Diego, S., Lucena Soto, J.M., Reyes Rosa, J.C., Florencio Bellido, F.J. y Candau Chacón, P. (1999). The presence of glutamate dehydrogenase is a selective advantage for the cyanobacterium Synechocystis sp. strain PCC 6803 under nonexponential growth conditions. Journal of Bacteriology, 181 (3), 808-813.
Tamaño: 110.7Kb
Formato: PDF

URI: http://hdl.handle.net/11441/63089

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones