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Modifying RANKL/OPG mRNA expression in differentiating and growing human primary osteoblasts

Opened Access Modifying RANKL/OPG mRNA expression in differentiating and growing human primary osteoblasts

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Autor: Giner García, Mercedes
Montoya-García, María-José
Vázquez-Gámez, MA
Ríos Moreno, María José
Moruno García, Rosa María
Miranda García, María José
Pérez Cano, Ramón
Departamento: Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica
Universidad de Sevilla. Departamento de Medicina
Fecha: 2008-12-01
Tipo de documento: Artículo
Resumen: The OPG / RANKL system in primary cultures of human osteoblasts has been studied by di ff erent authors. However, very few studies have been performed on gene expression of RANKL and OPG at di ff erent stages of maturation on human osteoblast cultures.The e ff ect of 17-estradiol and 1,25dihydroxyvitamin D3 on the OPG / RANKL system is not known during the di ff erent states of cellular maturation. In this work we quantifi ed OPG and RANKL protein levels (ELISA) and the mRNA of OPG, RANKL, collagen type I, alkaline phosphatase, and osteocalcin (semi-quantita-tive RT-PCR) in human osteoblasts. We analyzed these in basal conditions and after incubation with 17- -estradiol and 1,25dihydroxyvitamin D3 in the first and second phases. We found that OPG secretion and expression levels increased throughout cellular growth.RANKL proteins were detected only in the first stage, and the expression increased throughout the first phase. Thus, the RAN...
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Tamaño: 740.8Kb
Formato: PDF

URI: http://hdl.handle.net/11441/61072

DOI: 10.1055/s-0028-1082083

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