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dc.creatorGiner García, Mercedeses
dc.creatorMontoya García, María Josées
dc.creatorVázquez Gámez, María de los Ángeleses
dc.creatorRíos Moreno, María Josées
dc.creatorMoruno García, Rosa Maríaes
dc.creatorMiranda García, María Josées
dc.creatorPérez Cano, Ramónes
dc.date.accessioned2017-06-07T09:54:23Z
dc.date.available2017-06-07T09:54:23Z
dc.date.issued2008-12-01
dc.identifier.issn00185043es
dc.identifier.urihttp://hdl.handle.net/11441/61072
dc.description.abstractThe OPG / RANKL system in primary cultures of human osteoblasts has been studied by di ff erent authors. However, very few studies have been performed on gene expression of RANKL and OPG at di ff erent stages of maturation on human osteoblast cultures.The e ff ect of 17-estradiol and 1,25dihydroxyvitamin D3 on the OPG / RANKL system is not known during the di ff erent states of cellular maturation. In this work we quantifi ed OPG and RANKL protein levels (ELISA) and the mRNA of OPG, RANKL, collagen type I, alkaline phosphatase, and osteocalcin (semi-quantita-tive RT-PCR) in human osteoblasts. We analyzed these in basal conditions and after incubation with 17- -estradiol and 1,25dihydroxyvitamin D3 in the first and second phases. We found that OPG secretion and expression levels increased throughout cellular growth.RANKL proteins were detected only in the first stage, and the expression increased throughout the first phase. Thus, the RANKL / OPG ratio was higher in immature osteoblasts than in mature osteoblasts. The evolution of RANKL gene expression was related to collagen I and alkaline phosphatase, while OPG was related to osteocalcin. We observed no modi fi cations after estradiol and 1,25dihydroxyvitamin D3 treatment. Our results suggest that the OB is a positive stimulator at precocious stages of diff erentiation on osteoclastogenic modulates.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherGeorg Thiemees
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHuman osteoblast culturees
dc.subject17- -estradioles
dc.subject1,25dihydroxyvitamin D3es
dc.subjectOsteoporosises
dc.subjectOsteoarthritises
dc.titleModifying RANKL/OPG mRNA expression in differentiating and growing human primary osteoblastses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Citología e Histología Normal y Patológicaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.identifier.doi10.1055/s-0028-1082083es
idus.format.extent6es
dc.journaltitleHormone and Metabolic Researches
dc.publication.volumen40es
dc.publication.issue12es
dc.publication.initialPage869es
dc.publication.endPage874es

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional