Artículo
A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis
Autor/es | Juárez, Raquel
Ruiz Pérez, José Francisco McElhinny, Stephanie A Nick Ramsden, Dale A. Blanco, Luis |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2006 |
Fecha de depósito | 2017-02-07 |
Publicado en |
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Resumen | Human DNA polymerase mu (Polm) is a family X member that has terminal transferase activity but, in spite of a non-orthodox selection of the template information, displays its maximal catalytic efficiency in DNA-templated ... Human DNA polymerase mu (Polm) is a family X member that has terminal transferase activity but, in spite of a non-orthodox selection of the template information, displays its maximal catalytic efficiency in DNA-templated reactions. As terminal deoxynuc- leotidyl transferase (TdT), Polm has a specific loop (loop1) that could provide this enzyme with its ter- minal transferase activity. When loop1 was deleted, human Polm lacked TdT activity but improved DNA- binding and DNA template-dependent polymer- ization. Interestingly, when loop1 from TdT was inserted in Polm (substituting its cognate loop1), the resulting chimaera displayed TdT activity, preferen- tially inserting dGTP residues, but had a strongly reduced template-dependent polymerization activity. Therefore, a specialized loop in Polm, that could adopt alternative conformations, appears to provide this enzyme with a dual capacity: (i) template independency to create new DNA information, in which loop1 would have an active role by acting as a ‘pseudotemplate’; (ii) template-dependent polymer- ization, in which loop1 must allow binding of the template strand. Recent in vivo and in vitro data suggest that such a dual capacity could be advantageous to resolve microhomology-mediated end-joining reactions |
Cita | Juárez, R.E., Ruiz Pérez, J.F., McElhinny, S.A.N., Ramsden, D.A. y Blanco, L. (2006). A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis. Nucleic Acids Research, 34 (16), 4572-4582. |
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