dc.creator | Juárez, Raquel | es |
dc.creator | Ruiz Pérez, José Francisco | es |
dc.creator | McElhinny, Stephanie A Nick | es |
dc.creator | Ramsden, Dale A. | es |
dc.creator | Blanco, Luis | es |
dc.date.accessioned | 2017-02-07T13:31:06Z | |
dc.date.available | 2017-02-07T13:31:06Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Juárez, R.E., Ruiz Pérez, J.F., McElhinny, S.A.N., Ramsden, D.A. y Blanco, L. (2006). A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis. Nucleic Acids Research, 34 (16), 4572-4582. | |
dc.identifier.issn | 0305-1048 | es |
dc.identifier.uri | http://hdl.handle.net/11441/53785 | |
dc.description.abstract | Human DNA polymerase mu (Polm) is a family X member that has terminal transferase activity but, in spite of a non-orthodox selection of the template information, displays its maximal catalytic efficiency in DNA-templated reactions. As terminal deoxynuc- leotidyl transferase (TdT), Polm has a specific loop (loop1) that could provide this enzyme with its ter- minal transferase activity. When loop1 was deleted, human Polm lacked TdT activity but improved DNA- binding and DNA template-dependent polymer- ization. Interestingly, when loop1 from TdT was inserted in Polm (substituting its cognate loop1), the resulting chimaera displayed TdT activity, preferen- tially inserting dGTP residues, but had a strongly reduced template-dependent polymerization activity. Therefore, a specialized loop in Polm, that could adopt alternative conformations, appears to provide this enzyme with a dual capacity: (i) template independency to create new DNA information, in which loop1 would have an active role by acting as a ‘pseudotemplate’; (ii) template-dependent polymer- ization, in which loop1 must allow binding of the template strand. Recent in vivo and in vitro data suggest that such a dual capacity could be advantageous to resolve microhomology-mediated end-joining reactions | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic Acids Research, 34 (16), 4572-4582. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.publisherversion | 10.1093/nar/gkl457 | es |
idus.format.extent | 11 p. | es |
dc.journaltitle | Nucleic Acids Research | es |
dc.publication.volumen | 34 | es |
dc.publication.issue | 16 | es |
dc.publication.initialPage | 4572 | es |
dc.publication.endPage | 4582 | es |