dc.creator | Gutiérrez-Praena, Daniel | es |
dc.creator | Campos, Alexandre | es |
dc.creator | Azevedo, Joana | es |
dc.creator | Neves, Joana | es |
dc.creator | Freitas, Marisa | es |
dc.creator | Guzmán Guillén, Remedios | es |
dc.creator | Cameán Fernández, Ana María | es |
dc.creator | Renaut, Jenny | es |
dc.creator | Vasconcelos, Vitor | es |
dc.date.accessioned | 2016-10-07T12:29:00Z | |
dc.date.available | 2016-10-07T12:29:00Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Gutiérrez Praena, D., Campos, A., Azevedo, J., Neves, J., Freitas, M., Guzmán Guillén, R.,...,Vasconcelos, V. (2014). Exposure of lycopersicon esculentum to microcystin-LR: effects in the leaf proteome and toxin translocation from water to leaves and fruits. Toxins, 6, 1837-1854. | |
dc.identifier.issn | 2072-6651 | es |
dc.identifier.uri | http://hdl.handle.net/11441/47236 | |
dc.description.abstract | Natural toxins such as those produced by freshwater cyanobacteria have been
regarded as an emergent environmental threat. However, the impact of these water
contaminants in agriculture is not yet fully understood. The aim of this work was to
investigate microcystin-LR (MC-LR) toxicity in Lycopersicon esculentum and the toxin
accumulation in this horticultural crop. Adult plants (2 month-old) grown in a greenhouse
environment were exposed for 2 weeks to either pure MC-LR (100 μg/L) or Microcystis
aeruginosa crude extracts containing 100 μg/L MC-LR. Chlorophyll fluorescence was
measured, leaf proteome investigated with two-dimensional gel electrophoresis and Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF)/TOF, and
toxin bioaccumulation assessed by liquid chromatography-mass spectrometry (LC-MS)/MS.
Variations in several protein markers (ATP synthase subunits, Cytochrome b6-f complex
iron-sulfur, oxygen-evolving enhancer proteins) highlight the decrease of the capacity of
plants to synthesize ATP and to perform photosynthesis, whereas variations in other proteins
(ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and ribose-5-phosphate
isomerase) suggest an increase of carbon fixation and decrease of carbohydrate metabolism
reactions in plants exposed to pure MC-LR and cyanobacterial extracts, respectively. MC-LR
was found in roots (1635.21 μg/kg fw), green tomatoes (5.15–5.41 μg/kg fw), mature
tomatoes (10.52–10.83 μg/kg fw), and leaves (12,298.18 μg/kg fw). The results raise
concerns relative to food safety and point to the necessity of monitoring the bioaccumulation
of water toxins in agricultural systems affected by cyanotoxin contamination | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Toxins, 6, 1837-1854. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Lycopersicon esculentum | es |
dc.subject | Microcystin | es |
dc.subject | Phytotoxicity | es |
dc.subject | Bioaccumulation | es |
dc.subject | Proteomics | es |
dc.title | Exposure of lycopersicon esculentum to microcystin-LR: effects in the leaf proteome and toxin translocation from water to leaves and fruits | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.relation.publisherversion | 10.3390/toxins6061837 | es |
dc.identifier.doi | 10.3390/toxins6061837 | es |
idus.format.extent | 18 p. | es |
dc.journaltitle | Toxins | es |
dc.publication.volumen | 6 | es |
dc.publication.initialPage | 1837 | es |
dc.publication.endPage | 1854 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/47236 | |