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A Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Route

Opened Access A Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Route
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Autor: Muñiz Guinea, Manuel
Alonso, Manuel
Hidalgo Jiménez, Josefina
Velasco López, Ángel
Departamento: Universidad de Sevilla. Departamento de Biología Celular
Fecha: 1996
Publicado en: The Journal of Biological Chemistry, 271(48, Issue of November 29), 30935–30941
Tipo de documento: Artículo
Resumen: The influence of protein kinase A activity on transport of newly synthesized vesicular stomatitis virus G glycoprotein along the exocytic pathway was examined. Transport of vesicular stomatitis virus G glycoprotein to the cell surface was inhibited by N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), a selective inhibitor of protein kinase A. This block occurred at the exit of the Golgi complex, whereas transport through the Golgi compartments or from the endoplasmic reticulum to the Golgi was decreased in the presence of H-89. As judged by immunofluorescence endoplasmic reticulum to Golgi transport was accelerated in cells incubated with activators of protein kinase A such as isobutylmethylxanthine (IBMX) or forskolin (FK). Treatment with IBMX and FK also increased transport from the trans-Golgi network to the cell surface. During incubation with IBMX and FK, the organization of the Golgi complex was altered showing intercisternae fusion and miscom...
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URI: http://hdl.handle.net/11441/40673

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