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dc.creatorMuñiz Guinea, Manueles
dc.creatorAlonso, Manueles
dc.creatorHidalgo Jiménez, Josefinaes
dc.creatorVelasco López, Ángeles
dc.date.accessioned2016-05-03T11:52:53Z
dc.date.available2016-05-03T11:52:53Z
dc.date.issued1996
dc.identifier.citationMuñiz Guinea, M., Alonso, M., Hidalgo Jiménez, J. y Velasco López, Á. (1996). A Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Route. The Journal of Biological Chemistry, 271 (48), 30935.
dc.identifier.issn1083-351Xes
dc.identifier.urihttp://hdl.handle.net/11441/40673
dc.description.abstractThe influence of protein kinase A activity on transport of newly synthesized vesicular stomatitis virus G glycoprotein along the exocytic pathway was examined. Transport of vesicular stomatitis virus G glycoprotein to the cell surface was inhibited by N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), a selective inhibitor of protein kinase A. This block occurred at the exit of the Golgi complex, whereas transport through the Golgi compartments or from the endoplasmic reticulum to the Golgi was decreased in the presence of H-89. As judged by immunofluorescence endoplasmic reticulum to Golgi transport was accelerated in cells incubated with activators of protein kinase A such as isobutylmethylxanthine (IBMX) or forskolin (FK). Treatment with IBMX and FK also increased transport from the trans-Golgi network to the cell surface. During incubation with IBMX and FK, the organization of the Golgi complex was altered showing intercisternae fusion and miscompartmentalization of resident proteins. These structural changes affected both the kinetics of acquisition of endoglycosidase H resistance and transport activities. These data support a differential regulatory role for protein kinase A in different transport steps along the exocytic pathway. In particular, transport from the trans-Golgi network to the cell surface was dependent on protein kinase A activity. In addition, the results suggest the involvement of this enzyme on the maintenance of the Golgi complex organization.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherThe American Society for Biochemistry and Molecular Biologyes
dc.relation.ispartofThe Journal of Biological Chemistry, 271 (48), 30935.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleA Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Routees
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares
dc.relation.publisherversionhttp://www.jbc.org/content/271/48/30935.full.pdf+htmles
idus.format.extent8es
dc.journaltitleThe Journal of Biological Chemistry
dc.publication.volumen271
dc.publication.issue48
dc.publication.initialPage30935
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/40673

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