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Trimeric G proteins regulate the cytosol-induced redistribution of Golgi enzymes into the endoplasmic reticulum

Opened Access Trimeric G proteins regulate the cytosol-induced redistribution of Golgi enzymes into the endoplasmic reticulum
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Autor: Hidalgo Jiménez, Josefina
Muñiz Guinea, Manuel
Velasco López, Ángel
Departamento: Universidad de Sevilla. Departamento de Biología Celular
Fecha: 1995
Publicado en: Journal of Cell Science, 108 (4), 1805-1815
Tipo de documento: Artículo
Resumen: Streptolysin O-permeabilized cells incubated with a high concentration (5-10 mg/ml) of cytosolic proteins and ATPgenerating system exhibit redistribution into the endoplasmic reticulum (ER) of Golgi integral proteins (mannosidase II, galactosyltransferase, TGN 38), detected by immunofluorescence. In addition, mannosidase II is detected in the ER of cells exposed to a high concentration of cytosolic proteins and processed for immunolectron microscopy by immunoperoxidase. The redistribution process requires ATP and is not affected by previous microtubule depolymerization. Ultrastructural observations indicate that Golgi disassembly occurs by budding of coated vesicles. This stage of the process is inhibited by GTPgS, AlF(3-5), transducin bg subunits, and mastoparan, indicating the involvement of trimeric G proteins. At a later stage, vesicles lose their coats and fuse with the ER. This part of the process does not occur in cells incubated at either 15°C or 20°C, or expo...
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Tamaño: 918.3Kb
Formato: PDF

URI: http://hdl.handle.net/11441/40022

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