dc.creator | Ramos Morales, Francisco | |
dc.creator | Infante, Carlos R. | |
dc.creator | Fedriani Iriso, Concepción | |
dc.creator | Bornens, Michel | |
dc.creator | Ríos Sánchez, Rosa María | |
dc.date.accessioned | 2016-03-01T13:03:02Z | |
dc.date.available | 2016-03-01T13:03:02Z | |
dc.date.issued | 1999 | |
dc.identifier.citation | Ramos Morales, F., Infante, C.R., Fedriani Iriso, C., Bornens, M. y Ríos Sánchez, R.M. (1999). Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein. | |
dc.identifier.issn | 0021-9525 | es |
dc.identifier.uri | http://hdl.handle.net/11441/36688 | |
dc.description.abstract | We report that a peripheral Golgi protein
with a molecular mass of 210 kD localized at the cis-
Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C.
Antony, M.C. Boissier, J.C. Homberg, and M. Bornens.
1994.
J. Cell Biol.
125:997Ð1013) is a microtubule-binding
protein that associates in situ with a subpopulation
of stable microtubules. Interaction of this protein, now
called GMAP-210, for Golgi microtubule-associated
protein 210, with microtubules in vitro is direct, tight
and nucleotide-independent. Biochemical analysis further
suggests that GMAP-210 specifically binds to
microtubule ends. The full-length cDNA encoding
GMAP-210 predicts a protein of 1,979 amino acids with
a very long central coiled-coil domain. Deletion analyses
in vitro show that the COOH terminus of GMAP-
210 binds to microtubules whereas the NH
2
terminus
binds to Golgi membranes. Overexpression of GMAP-
210Ðencoding cDNA induced a dramatic enlargement
of the Golgi apparatus and perturbations in the microtubule
network. These effects did not occur when a mutant
lacking the COOH-terminal domain was expressed.
When transfected in fusion with the green
fluorescent protein, the NH
2
-terminal domain associated
with the cis-Golgi network whereas the COOHterminal
microtubule-binding domain localized at the
centrosome. Altogether these data support the view
that GMAP-210 serves to link the cis-Golgi network to
the minus ends of centrosome-nucleated microtubules.
In addition, this interaction appears essential for ensuring
the proper morphology and size of the Golgi apparatus | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Rockefeller University Press | es |
dc.relation.ispartof | null | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | autoantigens | es |
dc.subject | Golgi apparatus | es |
dc.subject | microtubule- associated proteins | es |
dc.subject | microtubules | es |
dc.subject | centrosome | es |
dc.title | Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | http://jcb.rupress.org/content/145/1/83.full.pdf+html | es |
dc.relation.publisherversion | http://dx.doi.org/10.1083/jcb.145.1.83 | |
dc.identifier.doi | 10.1083/jcb.145.1.83 | |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/36688 | |