Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein
|Author/s||Ramos Morales, Francisco
Infante, Carlos R.
Fedriani Iriso, Concepción
Ríos Sánchez, Rosa María
|Department||Universidad de Sevilla. Departamento de Genética
Universidad de Sevilla. Departamento de Microbiología
|Abstract||We report that a peripheral Golgi protein
with a molecular mass of 210 kD localized at the cis-
Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C.
Antony, M.C. Boissier, J.C. Homberg, and M. Bornens.
J. Cell ...
We report that a peripheral Golgi protein with a molecular mass of 210 kD localized at the cis- Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C. Antony, M.C. Boissier, J.C. Homberg, and M. Bornens. 1994. J. Cell Biol. 125:997Ð1013) is a microtubule-binding protein that associates in situ with a subpopulation of stable microtubules. Interaction of this protein, now called GMAP-210, for Golgi microtubule-associated protein 210, with microtubules in vitro is direct, tight and nucleotide-independent. Biochemical analysis further suggests that GMAP-210 specifically binds to microtubule ends. The full-length cDNA encoding GMAP-210 predicts a protein of 1,979 amino acids with a very long central coiled-coil domain. Deletion analyses in vitro show that the COOH terminus of GMAP- 210 binds to microtubules whereas the NH 2 terminus binds to Golgi membranes. Overexpression of GMAP- 210Ðencoding cDNA induced a dramatic enlargement of the Golgi apparatus and perturbations in the microtubule network. These effects did not occur when a mutant lacking the COOH-terminal domain was expressed. When transfected in fusion with the green fluorescent protein, the NH 2 -terminal domain associated with the cis-Golgi network whereas the COOHterminal microtubule-binding domain localized at the centrosome. Altogether these data support the view that GMAP-210 serves to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. In addition, this interaction appears essential for ensuring the proper morphology and size of the Golgi apparatus
|Citation||Ramos Morales, F., Infante, C.R., Fedriani Iriso, C., Bornens, M. y Ríos Sánchez, R.M. (1999). Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein.|