Mostrar el registro sencillo del ítem

Artículo

dc.creatorRuiz Pérez, José Francisco
dc.creatorGómez González, Belén
dc.creatorAguilera López, Andrés
dc.date.accessioned2015-10-05T11:38:32Z
dc.date.available2015-10-05T11:38:32Z
dc.date.issued2009
dc.identifier.citationRuiz Pérez, J.F., Gómez González, B. y Aguilera López, A. (2009). Chromosomal translocations caused by either Pol32-dependent or Pol32-independent triparental break-induced replication. Molecular and Cellular Biology, 29 (20), 5441-5454.
dc.identifier.issn1098-5549es
dc.identifier.issn0270-7306es
dc.identifier.urihttp://hdl.handle.net/11441/29153
dc.description.abstractDouble-strand breaks (DSBs) are harmful DNA lesions that can generate chromosomal rearrangements or chromosome losses if not properly repaired. Despite their association with a number of genetic diseases and cancer, the mechanisms by which DSBs cause rearrangements remain unknown. Using a newly developed experimental assay for the analysis of translocations occurring between two chromosomes in Saccharomyces cerevisiae, we found that a single DSB located on one chromosome uses a short homologous sequence found in a third chromosome as a bridge to complete DSB repair, leading to chromosomal translocations. Such translocations are dramatically reduced when the short homologous sequence on the third chromosome is deleted. Translocations rely on homologous recombination (HR) proteins, such as Rad51, Rad52, and Rad59, as well as on the break-induced replication-specific protein Pol32 and on Srs2, but not on Ku70. Our results indicate that a single chromosomal DSB efficiently searches for short homologous sequences throughout the genome for its repair, leading to triparental translocations between heterologous chromosomes. Given the abundance of repetitive DNA in eukaryotic genomes, the results of this study open the possibility that HR rather than nonhomologous end joining may be a major source of chromosomal translocations.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofMolecular and Cellular Biology, 29 (20), 5441-5454.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGenees
dc.subjectHomoloGenees
dc.titleChromosomal translocations caused by either Pol32-dependent or Pol32-independent triparental break-induced replicationen
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.publisherversionhttp://dx.doi.org/10.1128/MCB.00256-09es
dc.identifier.doi10.1128/MCB.00256-09es
dc.journaltitleMolecular and Cellular Biologyes
dc.publication.volumen29es
dc.publication.issue20es
dc.publication.initialPage5441es
dc.publication.endPage5454es
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/29153

FicherosTamañoFormatoVerDescripción
Mol. Cell. Biol.-2009-Ruiz-544 ...1.777MbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional