Repositorio de producción científica de la Universidad de Sevilla

The Npl3 hnRNP prevents R-loop-mediated transcription–replication conflicts and genome instability

Opened Access The Npl3 hnRNP prevents R-loop-mediated transcription–replication conflicts and genome instability

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Santos Pereira, José María
Herrero, Ana B.
García Rubio, María Luisa
Marín Rodríguez, Antonio
Moreno, Sergio
Aguilera López, Andrés
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2013
Publicado en: Genes & development, 27(22), 2445-2458
Tipo de documento: Artículo
Resumen: Transcription is a major obstacle for replication fork (RF) progression and a cause of genome instability. Part of this instability is mediated by cotranscriptional R loops, which are believed to increase by suboptimal assembly of the nascent messenger ribonucleoprotein particle (mRNP). However, no clear evidence exists that heterogeneous nuclear RNPs (hnRNPs), the basic mRNP components, prevent R-loop stabilization. Here we show that yeast Npl3, the most abundant RNA-binding hnRNP, prevents R-loop-mediated genome instability. npl3Δ cells show transcription-dependent and R-loop-dependent hyperrecombination and genome-wide replication obstacles as determined by accumulation of the Rrm3 helicase. Such obstacles preferentially occur at long and highly expressed genes, to which Npl3 is preferentially bound in wild-type cells, and are reduced by RNase H1 overexpression. The resulting replication stress confers hypersensitivity to double-strand break-inducing agents. Therefore, our work dem...
[Ver más]
Tamaño: 1.388Mb
Formato: PDF

URI: http://hdl.handle.net/11441/29092

DOI: http://dx.doi.org/10.1101/gad.229880.113

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones