Artículo
Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system
Autor/es | Stuckey, Ruth
García Rodriguez, Néstor Aguilera López, Andrés Wellinger, Ralf Erik |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2015 |
Fecha de depósito | 2015-10-01 |
Publicado en |
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Resumen | DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset ... DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by DNA polymerase-α and its intrinsic RNA primase activity. Here, we describe a novel origin-independent replication process that is mediated by transcription. RNA polymerase I transcription constraints lead to persistent RNA:DNA hybrids (R-loops) that prime replication in the ribosomal DNA locus. Our results suggest that eukaryotic genomes have developed tools to prevent R-loop–mediated replication events that potentially contribute to copy number variation, particularly relevant to carcinogenesis. |
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PNAS-2015-Stuckey-5779-84.pdf | 1.143Mb | [PDF] | Ver/ | |