Repositorio de producción científica de la Universidad de Sevilla

Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system

 

Advanced Search
 
Opened Access Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system
Cites

Show item statistics
Icon
Export to
Author: Stuckey, Ruth
García Rodriguez, Néstor
Aguilera López, Andrés
Wellinger, Ralf Erik
Department: Universidad de Sevilla. Departamento de Genética
Date: 2015
Published in: Proceedings of the National Academy of Sciences, 112(18), 5779-5784
Document type: Article
Abstract: DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by DNA polymerase-α and its intrinsic RNA primase activity. Here, we describe a novel origin-independent replication process that is mediated by transcription. RNA polymerase I transcription constraints lead to persistent RNA:DNA hybrids (R-loops) that prime replication in the ribosomal DNA locus. Our results suggest that eukaryotic genomes have developed tools to prevent R-loop–mediated replication events that potentially contribute to copy number variation, particularly relevant to carcinogenesis.
Size: 1.143Mb
Format: PDF

URI: http://hdl.handle.net/11441/29075

DOI: http://dx.doi.org/10.1073/pnas.1501769112

See editor´s version

This work is under a Creative Commons License: 
Attribution-NonCommercial-NoDerivatives 4.0 Internacional

This item appears in the following Collection(s)