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Mostrando ítems 1-10 de 16
Artículo
Ceramide chain length–dependent protein sorting into selective endoplasmic reticulum exit sites
(American Association for the Advancement of Science, 2020)
Protein sorting in the secretory pathway is crucial to maintain cellular compartmentalization and homeostasis. In addition to coat-mediated sorting, the role of lipids in driving protein sorting during secretory transport ...
Artículo
Determination of the lipid composition of the GPI anchor
(PLOS, 2021)
In eukaryotic cells, a subset of cell surface proteins is attached by the glycolipid glycosylphosphatidylinositol (GPI) to the external leaflet of the plasma membrane where they play important roles as enzymes, receptors, ...
Artículo
A Role for Lipids in Protein Sorting?
(Swiss Chemical Society, 2021)
Lipid and protein diversity provides structural and functional identity to the membrane compartments that define the eukaryotic cell. This compositional heterogeneity is maintained by the secretory pathway, which feeds ...
Artículo
The ceramide synthase subunit lac1 regulates cell growth and size in fission yeast
(Multidisciplinary Digital Publishing Institute (MDPI), 2022)
Cell division produces two viable cells of a defined size. Thus, all cells require mechanisms to measure growth and trigger cell division when sufficient growth has occurred. Previous data suggest a model in which growth ...
Artículo
Structural analysis of the GPI glycan
(PLOS, 2021)
Glycosylphosphatidylinositol (GPI) anchoring of proteins is an essential post-translational modification in all eukaryotes that occurs at the endoplasmic reticulum (ER) and serves to deliver GPI-anchored proteins (GPI-APs) ...
Artículo
Crosslinking assay to study a specific cargo-coat interaction through a transmembrane receptor in the secretory pathway
(Public Library of Science, 2022)
Intracellular trafficking through the secretory organelles depends on transient interactions between cargo proteins and transport machinery. Cytosolic coat protein complexes capture specific luminal cargo proteins for ...
Artículo
The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection
(Multidisciplinary Digital Publishing Institute (MDPI), 2021)
Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at ...
Artículo
The yeast p24 complex regulates gpi-anchored protein Transport and quality control by monitoring anchor remodeling
(American Society for Cell Biology, 2011)
Glycosylphosphatidylinositol (GPI)-anchored proteins are secretory proteins that are attached to the cell surface of eukaryotic cells by a glycolipid moiety. Once GPI anchoring has occurred in the lumen of the endoplasmic ...
Artículo
The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus
(Rockefeller University Press, 2008)
The p24 family members are transmembrane proteins assembled into heteromeric complexes that continuously cycle between the ER and the Golgi apparatus. These cargo proteins were assumed to play a structural role in COPI ...
Artículo
Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
(Multidisciplinary Digital Publishing Institute (MDPI), 2020)
The cellular mechanisms that ensure the selectivity and fidelity of secretory cargo protein transport from the endoplasmic reticulum (ER) to the Golgi are still not well understood. The p24 protein complex acts as a specific ...