The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection
|Sabido Bozo, Susana
Pérez Linero, Ana María
Manzano López, Javier
Rodríguez Gallardo, Sofía
Aguilera Romero, María Auxiliadora
Cortés Gómez, Alejandro
López Martín, Sergio
Wellinger, Ralf Erik
Muñiz Guinea, Manuel
|Universidad de Sevilla. Departamento de Biología Celular
|Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at ...
Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites. In yeast, Gea1 in the cis-Golgi and Gea2 in the medial-Golgi activate Arf1 to form COPIcoated vesicles for retrograde cargo sorting, whereas Sec7 generates clathrin/adaptorcoated vesicles at the trans-Golgi network (TGN) for forward cargo transport. A central question is how the same activated Arf1 protein manages to assemble different coats depending on the donor Golgi compartment. A previous study has postulated that the interaction between Gea1 and COPI would channel Arf1 activation for COPI vesicle budding. Here, we found that the p24 complex, a major COPI vesicle cargo, promotes the binding of Gea1 with COPI by increasing the COPI association to the membrane independently of Arf1 activation. Furthermore, the p24 complex also facilitates the interaction of Arf1 with its COPI effector. Therefore, our study supports a mechanism by which the p24 complex contributes to program Arf1 activation by Gea1 for selective COPI coat assembly at the cis-Golgi compartment.
|Sabido Bozo, S., Pérez Linero, A.M., Manzano López, J., Rodríguez Gallardo, S., Aguilera Romero, M.A., Cortés Gómez, A.,...,Muñiz Guinea, M. (2021). The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection. International Journal of Molecular Sciences, 22 (1), 423.