dc.creator | Rosillo Ramírez, María de los Ángeles | es |
dc.creator | Sánchez Hidalgo, Marina | es |
dc.creator | Cárdeno Galván, Ana | es |
dc.creator | Alarcón de la Lastra Romero, Catalina | es |
dc.date.accessioned | 2020-06-16T11:47:31Z | |
dc.date.available | 2020-06-16T11:47:31Z | |
dc.date.issued | 2011-10 | |
dc.identifier.citation | Rosillo Ramírez, M.d.l.Á., Sánchez Hidalgo, M., Cárdeno Galván, A. y Alarcón de la Lastra Romero, C. (2011). Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn’s disease. Biochemical Pharmacology, 82 (7), 737-745. | |
dc.identifier.issn | 0006-2952 | es |
dc.identifier.issn | 1873-2968 | es |
dc.identifier.uri | https://hdl.handle.net/11441/97892 | |
dc.description.abstract | Current epidemiological and experimental studies support a beneficial role of dietary polyphenols in several gastrointestinal diseases, including inflammatory bowel disease. The aim of this study was to gain a better understanding of the effects of a naturally occurring polyphenol, ellagic acid, present in some fruits such as pomegranate, raspberries and nuts among others, in an experimental murine model of Crohn's disease by intra-colonic administration of TNBS in rats. Analysis of the lesions were carried out by macroscopic and histological technics. Inflammation response was assessed by histology and myeloperoxidase activity. iNOS and COX-2 are upregulated by MAPKs and NF-κB nuclear transcription factor in intestinal epithelial cells thus, we determined the expression of iNOS, COX-2 and the involvement of the p38, JNK, ERK1/2 MAPKs and NF-κB signalling in the protective effect of EA by western blotting. Oral administration of EA (10–20 mg/kg) diminished the severity and extension of the intestinal injuries induced by TNBS although there was no observed a significant dose–response. In addition, EA increased mucus production in goblet cells in colon mucosa, decreased neutrophil infiltration and pro-inflammatory proteins COX-2 and iNOS overexpression. Also EA was capable of reducing the activation of p38, JNK and ERK1/2 MAPKs, preventing the inhibitory protein IκB-degradation and inducing an inhibition of the nuclear translocation level of p65 in colonic mucosa. In conclusion, EA reduces the damage in a rat model of Crohn's disease, alleviates the oxidative events and returns pro-inflammatory proteins expression to basal levels probably through MAPKs and NF-κB signalling pathways. | es |
dc.description.sponsorship | Junta de Andalucía y Ministerio de Ciencia e Innovación de España AGL 2008-02475 | es |
dc.format | application/pdf | es |
dc.format.extent | 8 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Biochemical Pharmacology, 82 (7), 737-745. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ellagic acid | es |
dc.subject | TNBS | es |
dc.subject | Oxidative stress | es |
dc.subject | COX-2 | es |
dc.subject | MAPK | es |
dc.subject | NF-κB | es |
dc.title | Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn’s disease | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Farmacología | es |
dc.relation.projectID | AGL 2008-02475 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.bcp.2011.06.043 | es |
dc.identifier.doi | 10.1016/j.bcp.2011.06.043 | es |
dc.journaltitle | Biochemical Pharmacology | es |
dc.publication.volumen | 82 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 737 | es |
dc.publication.endPage | 745 | es |
dc.contributor.funder | Junta de Andalucía | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Colegio Oficial de Farmacéuticos de Sevilla | es |