Artículo
Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn’s disease
Autor/es | Rosillo Ramírez, María de los Ángeles
![]() ![]() ![]() ![]() ![]() ![]() ![]() Sánchez Hidalgo, Marina ![]() ![]() ![]() ![]() ![]() ![]() ![]() Cárdeno Galván, Ana Alarcón de la Lastra Romero, Catalina ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Departamento | Universidad de Sevilla. Departamento de Farmacología |
Fecha de publicación | 2011-10 |
Fecha de depósito | 2020-06-16 |
Publicado en |
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Resumen | Current epidemiological and experimental studies support a beneficial role of dietary polyphenols in several gastrointestinal diseases, including inflammatory bowel disease. The aim of this study was to gain a better ... Current epidemiological and experimental studies support a beneficial role of dietary polyphenols in several gastrointestinal diseases, including inflammatory bowel disease. The aim of this study was to gain a better understanding of the effects of a naturally occurring polyphenol, ellagic acid, present in some fruits such as pomegranate, raspberries and nuts among others, in an experimental murine model of Crohn's disease by intra-colonic administration of TNBS in rats. Analysis of the lesions were carried out by macroscopic and histological technics. Inflammation response was assessed by histology and myeloperoxidase activity. iNOS and COX-2 are upregulated by MAPKs and NF-κB nuclear transcription factor in intestinal epithelial cells thus, we determined the expression of iNOS, COX-2 and the involvement of the p38, JNK, ERK1/2 MAPKs and NF-κB signalling in the protective effect of EA by western blotting. Oral administration of EA (10–20 mg/kg) diminished the severity and extension of the intestinal injuries induced by TNBS although there was no observed a significant dose–response. In addition, EA increased mucus production in goblet cells in colon mucosa, decreased neutrophil infiltration and pro-inflammatory proteins COX-2 and iNOS overexpression. Also EA was capable of reducing the activation of p38, JNK and ERK1/2 MAPKs, preventing the inhibitory protein IκB-degradation and inducing an inhibition of the nuclear translocation level of p65 in colonic mucosa. In conclusion, EA reduces the damage in a rat model of Crohn's disease, alleviates the oxidative events and returns pro-inflammatory proteins expression to basal levels probably through MAPKs and NF-κB signalling pathways. |
Agencias financiadoras | Junta de Andalucía Ministerio de Ciencia e Innovación (MICIN). España Colegio Oficial de Farmacéuticos de Sevilla |
Identificador del proyecto | AGL 2008-02475
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Cita | Rosillo Ramírez, M.d.l.Á., Sánchez Hidalgo, M., Cárdeno Galván, A. y Alarcón de la Lastra Romero, C. (2011). Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn’s disease. Biochemical Pharmacology, 82 (7), 737-745. |
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