Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material
|Lebrón Romero, José Antonio
Ostos Marcos, Francisco José
López López, Manuel
Moyá Morán, María Luisa
García Calderón, Clara Beatriz
García Calderón, Margarita
Peña Gómez, Mª José
Valle Rosado, Iván
Romero Balestra, Fernando
Huertas Sánchez, Pablo
López-Cornejo, María del Pilar
|Universidad de Sevilla. Departamento de Química Física
Instituto de Biomedicina de Sevilla (IBIS)
|Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid ...
Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)3]2+ complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction α was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between Kapp and α was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide's condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy.
|(Proyecto de Excelencia P12-FQM-1105, FQM-206 and FQM-274, and PI-0005-2018)
|Lebrón Romero, J.A., Ostos Marcos, F.J., López López, M., Moyá Morán, M.L., Sales, C., García, E.,...,López-Cornejo, M.P. (2020). Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material. Pharmaceutics, 12 (5), 482.