Show simple item record


dc.creatorOliveira, Flavioes
dc.creatorDíez-Quijada Jiménez, Leticiaes
dc.creatorTurkina, Maria
dc.creatorMoraes, Joao-Pauloes
dc.creatorJos Gallego, Ángeles Mencíaes
dc.creatorCameán Fernández, Ana Maríaes
dc.creatorBarreiro, Aldoes
dc.creatorAzevedo, Joanaes
dc.creatorVasconcelos, Vitores
dc.creatorMartins, José Carloses
dc.creatorCampos, Alexandrees
dc.description.abstractToxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYNes
dc.description.sponsorshipPortuguese Science Foundation and under the Projects MOREBIVALVES (PTDC/ASP-PES/31762/2017) and UID/Multi/04423/2013es
dc.description.sponsorshipNORTE 2020, Portugal 2020 and the European Union through the ERDF, and by FCT. Moreover, Project AGL2015-64558-Res
dc.description.sponsorshipMINECO/FEDER, UE, and the grant FPI (BES-2016–078773)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectMytilus galloprovincialises
dc.subjecttoxic cyanobacteriaes
dc.subjectshotgun proteomicses
dc.titlePhysiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporumes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legales
dc.relation.projectID(PTDC/ASP-PES/31762/2017) and UID/Multi/04423/2013es
dc.relation.projectIDProject AGL2015-64558-Res
dc.publication.issue3, art. 196es
dc.contributor.funderFundação para a Ciência e a Tecnologia. Portugales
dc.contributor.funderNORTE 2020es
dc.contributor.funderPORTUGAL 2020es
dc.contributor.funderEUROPEAN UNION, European Regional Development Fund (ERDF)es
dc.contributor.funderMinisterio de Economia, Industria y Competitividad (MINECO). Españaes
dc.contributor.funderEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)es

PUBtoxins-12-00196-v2.pdf3.161MbIcon   [PDF] View/Open  

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional