dc.creator | Espinar Marchena, Francisco José | es |
dc.creator | Rodríguez Galán, Olga | es |
dc.creator | Fernández Fernández, José | es |
dc.creator | Linnemann, Jan | es |
dc.creator | Cruz Díaz, Jesús de la | es |
dc.date.accessioned | 2018-12-20T12:41:59Z | |
dc.date.available | 2018-12-20T12:41:59Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Espinar Marchena, F.J., Rodríguez Galán, O., Fernández Fernández, J., Linnemann, J. y Cruz Díaz, J.d.l. (2018). Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Research, 46 (9), 4715-4732. | |
dc.identifier.issn | 1362-4962 | es |
dc.identifier.uri | https://hdl.handle.net/11441/81151 | |
dc.description.abstract | The contribution of most ribosomal proteins to ribo-
some synthesis has been quite well analysed in
Sac-
charomyces cerevisiae
. However, few yeast riboso-
mal proteins still await characterization. Herein, we
show that L14, an essential 60S ribosomal protein,
assembles in the nucleolus at an early stage into pre-
60S particles. Depletion of L14 results in a deficit in
60S subunits and defective processing of 27SA
2
and
27SA
3
to 27SB pre-rRNAs. As a result, 27S pre-rRNAs
are subjected to turnover and export of pre-60S par-
ticles is blocked. These phenotypes likely appear as
the direct consequence of the reduced pre-60S par-
ticle association not only of L14 upon its depletion
but also of a set of neighboring ribosomal proteins
located at the solvent interface of 60S subunits and
the adjacent region surrounding the polypeptide exit
tunnel. These pre-60S intermediates also lack some
essential
trans
-acting factors required for 27SB pre-
rRNA processing but accumulate practically all fac-
tors required for processing of 27SA
3
pre-rRNA. We
have also analysed the functional interaction be-
tween the eukaryote-specific carboxy-terminal exten-
sions of the neighboring L14 and L16 proteins. Our
results indicate that removal of the most distal parts
of these extensions cause slight translation alter-
ations in mature 60S subunits. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad (BFU2013-42958-P), (BFU2016-75352- P) | es |
dc.description.sponsorship | Deutsche Forschungs- gemeinschaft’ (SFB 960) | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic Acids Research, 46 (9), 4715-4732. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BFU2013-42958-P | es |
dc.relation.projectID | BFU2016-75352- P | es |
dc.relation.projectID | SFB 960 | es |
dc.relation.publisherversion | https://doi.org/10.1093/nar/gky123 | es |
dc.identifier.doi | 10.1093/nar/gky123 | es |
idus.format.extent | 17 | es |
dc.journaltitle | Nucleic Acids Research | es |
dc.publication.volumen | 46 | es |
dc.publication.issue | 9 | es |
dc.publication.initialPage | 4715 | es |
dc.publication.endPage | 4732 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |
dc.contributor.funder | Deutsche Forschungsgemeinschaft / German Research Foundation (DFG) | |
dc.description.awardwinning | Premio Anual Publicación Científica Destacada de la US. Facultad de Biología | |