Article
Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae
Author/s | Espinar Marchena, Francisco José
Rodríguez Galán, Olga Fernández Fernández, José Linnemann, Jan Cruz Díaz, Jesús de la ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Genética |
Date | 2018 |
Published in |
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Abstract | The contribution of most ribosomal proteins to ribo-
some synthesis has been quite well analysed in
Sac-
charomyces cerevisiae
. However, few yeast riboso-
mal proteins still await characterization. Herein, we
show ... The contribution of most ribosomal proteins to ribo- some synthesis has been quite well analysed in Sac- charomyces cerevisiae . However, few yeast riboso- mal proteins still await characterization. Herein, we show that L14, an essential 60S ribosomal protein, assembles in the nucleolus at an early stage into pre- 60S particles. Depletion of L14 results in a deficit in 60S subunits and defective processing of 27SA 2 and 27SA 3 to 27SB pre-rRNAs. As a result, 27S pre-rRNAs are subjected to turnover and export of pre-60S par- ticles is blocked. These phenotypes likely appear as the direct consequence of the reduced pre-60S par- ticle association not only of L14 upon its depletion but also of a set of neighboring ribosomal proteins located at the solvent interface of 60S subunits and the adjacent region surrounding the polypeptide exit tunnel. These pre-60S intermediates also lack some essential trans -acting factors required for 27SB pre- rRNA processing but accumulate practically all fac- tors required for processing of 27SA 3 pre-rRNA. We have also analysed the functional interaction be- tween the eukaryote-specific carboxy-terminal exten- sions of the neighboring L14 and L16 proteins. Our results indicate that removal of the most distal parts of these extensions cause slight translation alter- ations in mature 60S subunits. |
Funding agencies | Ministerio de Economía y Competitividad (MINECO). España Deutsche Forschungsgemeinschaft / German Research Foundation (DFG) |
Project ID. | BFU2013-42958-P
![]() BFU2016-75352- P ![]() SFB 960 ![]() |
Citation | Espinar Marchena, F.J., Rodríguez Galán, O., Fernández Fernández, J., Linnemann, J. y Cruz Díaz, J.d.l. (2018). Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Research, 46 (9), 4715-4732. |
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