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dc.creatorGuzmán Guillén, Remedioses
dc.creatorPrieto Ortega, Ana Isabeles
dc.creatorDíez-Quijada Jiménez, Leticiaes
dc.creatorLlana Ruiz-Cabello, Maríaes
dc.creatorCampos, Alexandrees
dc.creatorVasconcelos, Vitores
dc.creatorJos Gallego, Ángeles Mencíaes
dc.creatorCameán Fernández, Ana Maríaes
dc.date.accessioned2018-11-26T11:18:27Z
dc.date.available2018-11-26T11:18:27Z
dc.date.issued2018
dc.identifier.citationGuzmán Guillén, R., Prieto Ortega, A.I., Díez-Quijada Jiménez, L., Llana Ruiz-Cabello, M., Campos, A., Vasconcelos, V.,...,Cameán Fernández, A.M. (2018). New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS. Toxins, 10 (10), 1-16.
dc.identifier.issn2072-6651es
dc.identifier.urihttps://hdl.handle.net/11441/80517
dc.description.abstractCyanotoxins are a large group of noxious metabolites with different chemical structure and mechanisms of action, with a worldwide distribution, producing effects in animals, humans, and crop plants. When cyanotoxin-contaminated waters are used for the irrigation of edible vegetables, humans can be in contact with these toxins through the food chain. In this work, a method for the simultaneous detection of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR), Microcystin-YR (MC-YR), and Cylindrospermopsin (CYN) in lettuce has been optimized and validated, using a dual solid phase extraction (SPE) system for toxin extraction and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for analysis. Results showed linear ranges (5–50 ng g−1 f.w.), low values for limit of detection (LOD) (0.06–0.42 ng g−1 f.w.), and limit of quantification (LOQ) (0.16–0.91 ng g−1 f.w.), acceptable recoveries (41–93%), and %RSDIP values for the four toxins. The method proved to be robust for the three variables tested. Finally, it was successfully applied to detect these cyanotoxins in edible vegetables exposed to cyanobacterial extracts under laboratory conditions, and it could be useful for monitoring these toxins in edible vegetables for better exposure estimation in terms of risk assessment.es
dc.description.sponsorshipEspaña MINECO AGL2015-64558-Res
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofToxins, 10 (10), 1-16.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectmicrocystinses
dc.subjectcylindrospermopsines
dc.subjectmethod validationes
dc.subjectUPLC-MS/MSes
dc.subjectlettucees
dc.titleNew Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MSes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legales
dc.relation.projectIDAGL2015-64558-Res
dc.relation.publisherversionhttp://dx.doi.org/10.3390/toxins10100406es
dc.identifier.doi10.3390/toxins10100406es
idus.format.extent16 p.es
dc.journaltitleToxinses
dc.publication.volumen10es
dc.publication.issue10es
dc.publication.initialPage1es
dc.publication.endPage16es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España

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