dc.creator | Olmeda, Bárbara | es |
dc.creator | Umstead, Todd M. | es |
dc.creator | Silveyra, Patricia | es |
dc.creator | Pascual Bravo, Alberto | es |
dc.creator | López Barneo, José | es |
dc.creator | Phelps, David S. | es |
dc.creator | Floros, Joanna | es |
dc.creator | Pérez Gil, Jesús | es |
dc.date.accessioned | 2018-08-17T10:07:14Z | |
dc.date.available | 2018-08-17T10:07:14Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Olmeda, B., Umstead, T.M., Silveyra, P., Pascual Bravo, A., López Barneo, J., Phelps, D.S.,...,Pérez Gil, J. (2014). Effect of hypoxia on lung gene expression and proteomic profile: insights into the pulmonary surfactant response. Journal of Proteomics, 101, 179-191. | |
dc.identifier.issn | 1874-3919 | es |
dc.identifier.uri | https://hdl.handle.net/11441/78171 | |
dc.description.abstract | Exposure of lung to hypoxia has been previously reported to be associated with significant alterations in the protein content of bronchoalveolar lavage (BAL) and lung tissue. In the present work we have used a proteomic approach to describe the changes in protein complement induced by moderate long-term hypoxia (rats exposed to 10% O2 for 72h) in BAL and lung tissue, with a special focus on the proteins associated with pulmonary surfactant, which could indicate adaptation of this system to limited oxygen availability. The analysis of the general proteomic profile indicates a hypoxia-induced increase in proteins associated with inflammation both in lavage and lung tissue. Analysis at mRNA and protein levels revealed no significant changes induced by hypoxia on the content in surfactant proteins or their apparent oligomeric state. In contrast, we detected a hypoxia-induced significant increase in the expression and accumulation of hemoglobin in lung tissue, at both mRNA and protein levels, as well as an accumulation of hemoglobin both in BAL and associated with surface-active membranes of the pulmonary surfactant complex. Evaluation of pulmonary surfactant surface activity from hypoxic rats showed no alterations in its spreading ability, ruling out inhibition by increased levels of serum or inflammatory proteins. | es |
dc.description.sponsorship | Ministerio de Ciencia BIO2012-30733 | es |
dc.description.sponsorship | Ministerio de Ciencia CSD2007-00010 | es |
dc.description.sponsorship | Gobierno de la Comunidad de Madrid S2009MAT-1507 | es |
dc.description.sponsorship | National Institutes of Health NIH HL34788 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Proteomics, 101, 179-191. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | ABCA3 | es |
dc.subject | Air–liquid interface | es |
dc.subject | Hemoglobin | es |
dc.subject | Lung surfactant | es |
dc.subject | Surfactant proteins | es |
dc.title | Effect of hypoxia on lung gene expression and proteomic profile: insights into the pulmonary surfactant response | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.identifier.doi | 10.1016/j.jprot.2014.02.019 | es |
dc.contributor.group | Universidad de Sevilla. CTS516: Fisiología Celular y Biofísica | es |
idus.format.extent | 27 | es |
dc.journaltitle | Journal of Proteomics | es |
dc.publication.volumen | 101 | es |
dc.publication.initialPage | 179 | es |
dc.publication.endPage | 191 | es |