dc.creator | Fernández-Espejo, Emilio | es |
dc.creator | García Moreno, José Manuel | es |
dc.creator | Martín de Pablos, Ángel | es |
dc.creator | Chacón, José | es |
dc.date.accessioned | 2018-05-28T17:28:24Z | |
dc.date.available | 2018-05-28T17:28:24Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Fernández-Espejo, E., García Moreno, J.M., Martín de Pablos, Á. y Chacón, J. (2013). May the evaluation of nitrosative stress through selective increase of 3-nitrotyrosine proteins other than nitroalbumin and dominant tyrosine-125/136 nitrosylation of serum α-synuclein serve for diagnosis of sporadic Parkinson's disease?. Antioxidants & Redox Signaling, 19 (9), 912-918. | |
dc.identifier.issn | 1557-7716 | es |
dc.identifier.uri | https://hdl.handle.net/11441/75280 | |
dc.description.abstract | Nitrosative stress, where nitrosylation of tyrosine (Tyr) leading to 3-nitrotyrosine proteins or free 3-nitrotyrosine is the most prominent change, has been proposed as a pathogenic mechanism in Parkinson's disease (PD). Levels of 3-nitrotyrosine proteins in serum and cerebrospinal fluid (CSF) of patients with PD have not been studied. Nitrosative stress-induced protein changes in serum and CSF were analyzed in patients with PD (n=54) and controls (n=40). Herein, we demonstrate the presence of nitrosative stress in serum and CSF of patients with early PD leading to selective increase of 3-nitrotyrosine proteins other than nitroalbumin, without free 3-nitrotyrosine (Hoehn-Yahr stage 1, p<0.05; stage 2, p<0.01). Among 3-nitrotyrosine proteins, nitro-α-synuclein (N-αSyn) was detected in serum, not CSF, and the sites of Tyr nitrosylation were observed to be modified in patients with early PD. Thus, the intensity of nitrosylation of Tyr125/136 residues is enhanced (stage 1, p<0.05; stage 2, p<0.01), and that of the Tyr39 site is reduced (stage 1, p<0.05), and the ratio between both parameters (α-synuclein with nitrosylated tyrosines 125 and 136 [N-αSyn-Tyr125/136]:α-synuclein with nitrosylated tyrosine 39 [N-αSyn-Tyr39] ratio) is significantly higher in patients with early PD (p<0.01). These observations lead to the hypothesis that evaluating nitrosative stress through enhanced levels of 3-nitrotyrosine proteins in serum and CSF without changes in nitroalbumin, together with the profile of tyrosine nitrosylation of serum αSyn characterized by dominant nitrosylation of Tyr125/136, could serve for the diagnosis of sporadic PD. | es |
dc.description.sponsorship | Instituto de Salud Carlos III RD09/0076/00080 | es |
dc.description.sponsorship | Ministerio de Sanidad RETICS, RD06/ 001/002 | es |
dc.description.sponsorship | Ministerio de Sanidad RETICS, RD06/010/1007 | es |
dc.description.sponsorship | Unión Europea FEDER, European Fund for Regional Development | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Mary Ann Liebert, Inc. Publishers | es |
dc.relation.ispartof | Antioxidants & Redox Signaling, 19 (9), 912-918. | |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Nitrosative stress | es |
dc.subject | Parkinson's disease | es |
dc.title | May the evaluation of nitrosative stress through selective increase of 3-nitrotyrosine proteins other than nitroalbumin and dominant tyrosine-125/136 nitrosylation of serum α-synuclein serve for diagnosis of sporadic Parkinson's disease? | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.identifier.doi | 10.1089/ars.2013.5250 | es |
dc.contributor.group | Universidad de Sevilla. BIO127: Laboratorio de Neurofisiología y Neurología Molecular | es |
idus.format.extent | 7 | es |
dc.journaltitle | Antioxidants & Redox Signaling | es |
dc.publication.volumen | 19 | es |
dc.publication.issue | 9 | es |
dc.publication.initialPage | 912 | es |
dc.publication.endPage | 918 | es |