Show simple item record

Article

dc.creatorFernández-Espejo, Emilioes
dc.creatorGarcía Moreno, José Manueles
dc.creatorMartín de Pablos, Ángeles
dc.creatorChacón, Josées
dc.date.accessioned2018-05-28T17:28:24Z
dc.date.available2018-05-28T17:28:24Z
dc.date.issued2013
dc.identifier.citationFernández-Espejo, E., García Moreno, J.M., Martín de Pablos, Á. y Chacón, J. (2013). May the evaluation of nitrosative stress through selective increase of 3-nitrotyrosine proteins other than nitroalbumin and dominant tyrosine-125/136 nitrosylation of serum α-synuclein serve for diagnosis of sporadic Parkinson's disease?. Antioxidants & Redox Signaling, 19 (9), 912-918.
dc.identifier.issn1557-7716es
dc.identifier.urihttps://hdl.handle.net/11441/75280
dc.description.abstractNitrosative stress, where nitrosylation of tyrosine (Tyr) leading to 3-nitrotyrosine proteins or free 3-nitrotyrosine is the most prominent change, has been proposed as a pathogenic mechanism in Parkinson's disease (PD). Levels of 3-nitrotyrosine proteins in serum and cerebrospinal fluid (CSF) of patients with PD have not been studied. Nitrosative stress-induced protein changes in serum and CSF were analyzed in patients with PD (n=54) and controls (n=40). Herein, we demonstrate the presence of nitrosative stress in serum and CSF of patients with early PD leading to selective increase of 3-nitrotyrosine proteins other than nitroalbumin, without free 3-nitrotyrosine (Hoehn-Yahr stage 1, p<0.05; stage 2, p<0.01). Among 3-nitrotyrosine proteins, nitro-α-synuclein (N-αSyn) was detected in serum, not CSF, and the sites of Tyr nitrosylation were observed to be modified in patients with early PD. Thus, the intensity of nitrosylation of Tyr125/136 residues is enhanced (stage 1, p<0.05; stage 2, p<0.01), and that of the Tyr39 site is reduced (stage 1, p<0.05), and the ratio between both parameters (α-synuclein with nitrosylated tyrosines 125 and 136 [N-αSyn-Tyr125/136]:α-synuclein with nitrosylated tyrosine 39 [N-αSyn-Tyr39] ratio) is significantly higher in patients with early PD (p<0.01). These observations lead to the hypothesis that evaluating nitrosative stress through enhanced levels of 3-nitrotyrosine proteins in serum and CSF without changes in nitroalbumin, together with the profile of tyrosine nitrosylation of serum αSyn characterized by dominant nitrosylation of Tyr125/136, could serve for the diagnosis of sporadic PD.es
dc.description.sponsorshipInstituto de Salud Carlos III RD09/0076/00080es
dc.description.sponsorshipMinisterio de Sanidad RETICS, RD06/ 001/002es
dc.description.sponsorshipMinisterio de Sanidad RETICS, RD06/010/1007es
dc.description.sponsorshipUnión Europea FEDER, European Fund for Regional Developmentes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherMary Ann Liebert, Inc. Publisherses
dc.relation.ispartofAntioxidants & Redox Signaling, 19 (9), 912-918.
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectNitrosative stresses
dc.subjectParkinson's diseasees
dc.titleMay the evaluation of nitrosative stress through selective increase of 3-nitrotyrosine proteins other than nitroalbumin and dominant tyrosine-125/136 nitrosylation of serum α-synuclein serve for diagnosis of sporadic Parkinson's disease?es
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiología Médica y Biofísicaes
dc.identifier.doi10.1089/ars.2013.5250es
dc.contributor.groupUniversidad de Sevilla. BIO127: Laboratorio de Neurofisiología y Neurología Moleculares
idus.format.extent7es
dc.journaltitleAntioxidants & Redox Signalinges
dc.publication.volumen19es
dc.publication.issue9es
dc.publication.initialPage912es
dc.publication.endPage918es

FilesSizeFormatViewDescription
May the Evaluation of Nitrosative ...376.4KbIcon   [PDF] View/Open  

This item appears in the following collection(s)

Show simple item record

Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América
Except where otherwise noted, this item's license is described as: Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América