Article
Securin Is a Target of the UV Response Pathway in Mammalian Cells
Author/s | Romero Portillo, Francisco
Gil-Bernabé, Ana M. Sáez, Carmen Pintor Toro, José Antonio Tortolero García, María Dolores |
Department | Universidad de Sevilla. Departamento de Microbiología Universidad de Sevilla. Departamento de Anatomía Patológica |
Publication Date | 2004 |
Deposit Date | 2018-03-05 |
Published in |
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Abstract | All eukaryotic cells possess elaborate mechanisms to protect genome integrity and ensure survival after DNA damage, ceasing proliferation and granting time for DNA repair. Securin is an inhibitory protein that is bound to ... All eukaryotic cells possess elaborate mechanisms to protect genome integrity and ensure survival after DNA damage, ceasing proliferation and granting time for DNA repair. Securin is an inhibitory protein that is bound to a protease called Separase to inhibit sister chromatid separation until the onset of anaphase. At the metaphase-to-anaphase transition, Securin is degraded by the anaphase-promoting complex or cyclosome, and Separase contributes to the release of cohesins from the chromosome, allowing for the segregation of sister chromatids to opposite spindle poles. Here we provide evidence that human Securin (hSecurin) has a novel role in cell cycle arrest after exposure to UV light or ionizing radiation. In fact, irradiation downregulated the level of hSecurin protein, accelerating its degradation via the proteasome and reducing hSecurin mRNA translation, but the presence of hSecurin is necessary for cell proliferation arrest following UV treatment. Moreover, an alteration of UV-induced hSecurin downregulation could lead directly to the accumulation of DNA damage and the subsequent development of malignant tumors. |
Funding agencies | Ministerio de Ciencia y Tecnología (MCYT). España Junta de Andalucía |
Citation | Romero Portillo, F., Gil-Bernabé, A.M., Sáez, C., Pintor Toro, J.A. y Tortolero García, M.D. (2004). Securin Is a Target of the UV Response Pathway in Mammalian Cells. Molecular and Cellular Biology, 24 (7), 2720-2733. |
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