Artículo
Subtracting the sequence bias from partially digested MNase-seq data reveals a general contribution of TFIIS to nucleosome positioning
Autor/es | Gutiérrez Pozo, Gabriel
Millán Zambrano, Gonzalo Medina, Daniel A. Jordán Pla, Antonio Pérez Ortín, José E. Peñate Salas, Xenia Chávez de Diego, Sebastián |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2017 |
Fecha de depósito | 2018-02-09 |
Publicado en |
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Resumen | TFIIS stimulates RNA cleavage by RNA polymerase II and promotes the resolution of backtracking events. TFIIS acts in the chromatin context, but its contribution to the chromatin landscape has not yet been investigated. ... TFIIS stimulates RNA cleavage by RNA polymerase II and promotes the resolution of backtracking events. TFIIS acts in the chromatin context, but its contribution to the chromatin landscape has not yet been investigated. Co-transcriptional chromatin alterations include subtle changes in nucleosome positioning, like those expected to be elicited by TFIIS, which are elusive to detect. The most popular method to map nucleosomes involves intensive chromatin digestion by micrococcal nuclease (MNase). Maps based on these exhaustively digested samples miss any MNase-sensitive nucleosomes caused by transcription. In contrast, partial digestion approaches preserve such nucleosomes, but introduce noise due to MNase sequence preferences. A systematic way of correcting this bias for massively parallel sequencing experiments is still missing. |
Cita | Gutiérrez Pozo, G., Millán Zambrano, G., Medina, D.A., Jordán Pla, A., Pérez Ortín, .E., Peñate, X. y Chávez de Diego, S. (2017). Subtracting the sequence bias from partially digested MNase-seq data reveals a general contribution of TFIIS to nucleosome positioning. Epigenetics Chromatin, 10 (58), 1-22. |
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