dc.creator | Ríos Camacho, María Victoria | es |
dc.creator | Guzmán Guillén, Remedios | es |
dc.creator | Moreno Navarro, Isabel María | es |
dc.creator | Prieto Ortega, Ana Isabel | es |
dc.creator | Puerto Rodríguez, María | es |
dc.creator | Jos Gallego, Ángeles Mencía | es |
dc.creator | Cameán Fernández, Ana María | es |
dc.date.accessioned | 2016-10-07T12:27:49Z | |
dc.date.available | 2016-10-07T12:27:49Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Ríos Camacho, M.V., Guzmán Guillén, R., Moreno Navarro, I.M., Prieto Ortega, A.I., Puerto Rodríguez, M., Jos Gallego, Á.M. y Cameán Fernández, A.M. (2014). Influence of two depuration periods on the activity and transcription of antioxidant enzymes in tilapia exposed to repeated doses of Cylindrospermopsin under laboratory conditions. Toxins, 6, 1062-1079. | |
dc.identifier.issn | 2072-6651 | es |
dc.identifier.uri | http://hdl.handle.net/11441/47232 | |
dc.description.abstract | The cyanobacterial toxin Cylindrospermopsin (CYN), a potent protein synthesis
inhibitor, is increasingly being found in freshwater bodies infested by cyanobacterial
blooms worldwide. Moreover, it has been reported to be implicated in human intoxications
and animal mortality. Recently, the alteration of the activity and gene expression of some
glutathione related enzymes in tilapias (Oreochromis niloticus) exposed to a single dose of
CYN has been reported. However, little is known about the effects induced by repeated
doses of this toxin in tilapias exposed by immersion and the potential reversion of these
biochemical alterations after two different depuration periods (3 or 7 days). In the present
study, tilapias were exposed by immersion to repeated doses of a CYN-containing culture
of Aphanizomenon ovalisporum during 14 days, and then were subjected to depuration
periods (3 or 7 days) in clean water in order to examine the potential reversion of the
effects observed. The activity and relative mRNA expression by real-time polymerase
chain reaction (PCR) of the antioxidant enzymes glutathione peroxidase (GPx) and soluble
glutathione-S-transferases (sGST), and also the sGST protein abundance by Western blot
analysis were evaluated in liver and kidney of fish. Results showed significant alterations
in most of the parameters evaluated and their recovery after 3 days (GPx activity, sGST
relative abundance) or 7 days (GPx gene expression, sGST activity). These findings not only
confirm the oxidative stress effects produced in fish by cyanobacterial cells containing
CYN, but also show the effectiveness of depuration processes in mitigating the
CYN-containing culture toxic effects | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Toxins, 6, 1062-1079. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cylindrospermopsin | es |
dc.subject | Depuration | es |
dc.subject | Glutathione transferase | es |
dc.subject | Glutathione peroxidise | es |
dc.subject | Tilapia | es |
dc.title | Influence of two depuration periods on the activity and transcription of antioxidant enzymes in tilapia exposed to repeated doses of Cylindrospermopsin under laboratory conditions | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.relation.publisherversion | 10.3390/toxins6031062 | es |
dc.identifier.doi | 10.3390/toxins6031062 | es |
idus.format.extent | 18 p. | es |
dc.journaltitle | Toxins | es |
dc.publication.volumen | 6 | es |
dc.publication.initialPage | 1062 | es |
dc.publication.endPage | 1079 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/47232 | |