dc.creator | Gaillard, Hélène | |
dc.creator | Aguilera López, Andrés | |
dc.date.accessioned | 2015-10-06T09:10:10Z | |
dc.date.available | 2015-10-06T09:10:10Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Gaillard, H. y Aguilera López, A. (2008). A novel class of Mrna-containing cytoplasmic granules are produced in response to UV-irradiation. Molecular Biology of the Cell, 19 (11), 4980-4992. | es |
dc.identifier.issn | 1059-1524 | es |
dc.identifier.issn | 1939-4586 | es |
dc.identifier.uri | http://hdl.handle.net/11441/29191 | |
dc.description.abstract | Nucleic acids are substrates for different types of damage, but little is known about the fate of damaged RNAs. We addressed the existence of an RNA-damage response in yeast. The decay kinetics of GAL1p-driven mRNAs revealed a dose-dependent mRNA stabilization upon UV-irradiation that was not observed after heat or saline shocks, or during nitrogen starvation. UV-induced mRNA stabilization did not depend on DNA repair, damage checkpoint or mRNA degradation machineries. Notably, fluorescent in situ hybridization revealed that after UV-irradiation, polyadenylated mRNA accumulated in cytoplasmic foci that increased in size with time. In situ colocalization showed that these foci are not processing-bodies, eIF4E-, eIF4G-, and Pab1-containing bodies, stress granules, autophagy vesicles, or part of the secretory or endocytic pathways. These results point to the existence of a specific eukaryotic RNA-damage response, which leads to new polyadenylated mRNA-containing granules (UV-induced mRNA granules; UVGs). We propose that potentially damaged mRNAs, which may be deleterious to the cell, are temporarily stored in UVG granules to safeguard cell viability. | es |
dc.format | application/pdf | es |
dc.language.iso | spa | es |
dc.publisher | American Society for Cell Biology | es |
dc.relation.ispartof | Molecular Biology of the Cell, 19(11), 4980-4992 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cytoplasmic Granules | es |
dc.subject | Endocytosis | es |
dc.subject | Galactokinase | es |
dc.subject | Gene Expression Regulation, Fungal | es |
dc.subject | Heat-Shock Response | es |
dc.subject | Nitrogen | es |
dc.subject | Polyribosomes | es |
dc.subject | RNA Stability | es |
dc.subject | RNA, Messenger | es |
dc.subject | Saccharomyces cerevisiae | es |
dc.subject | Sodium Chloride | es |
dc.subject | Ultraviolet Rays | es |
dc.subject | Poly A | es |
dc.title | A novel class of Mrna-containing cytoplasmic granules are produced in response to UV-irradiation | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1091/mbc.E08-02-0193 | es |
dc.identifier.doi | http://dx.doi.org/10.1091/mbc.E08-02-0193 | es |
dc.journaltitle | Molecular Biology of the Cell | es |
dc.publication.volumen | 19 | es |
dc.publication.issue | 11 | es |
dc.publication.initialPage | 4980 | es |
dc.publication.endPage | 4992 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/29191 | |