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Artículo
Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2
dc.creator | Sorensen, Jakob B. | |
dc.creator | Fernández-Chacón, Rafael | |
dc.creator | Südhof, Thomas C. | |
dc.creator | Neher, Erwin | |
dc.date.accessioned | 2015-01-19T11:11:51Z | |
dc.date.available | 2015-01-19T11:11:51Z | |
dc.date.issued | 2003 | |
dc.identifier.issn | 1540-7748 | es |
dc.identifier.issn | 0022-1295 | es |
dc.identifier.uri | http://hdl.handle.net/11441/17816 | |
dc.description.abstract | We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2 +_ sensor for fast neurosecretion by analyzing the intracellular Ca 2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a mutated synaptotagmin C2A domain. The mutation (R233Q) causes a twofold increase in the K D of Ca 2 + - dependent phospholipid binding to the double C2A-C2B domain of synaptotagmin. Using photolysis of caged calcium and capacitance measurements we found that secretion from mutant cells had lower secretory rates, longer secretory delays, and a higher intracellular Ca 2 + -threshold for secretion due to a twofold increase in the apparent K D of the Ca 2 + sensor for fast exocytosis. Single amperometric fusion events were unchanged. We conclude that Ca 2 + -dependent phospholipid binding to synaptotagmin 1 mirrors the intracellular Ca 2 + dependence of exocytosis. | es |
dc.language.iso | eng | es |
dc.relation.ispartof | The Journal of general physiology, 122 (3), 265-276. | es |
dc.rights | Atribución-NoComercial-SinDerivadas 4.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Chromaffin cell | es |
dc.subject | Membrane fusion | en |
dc.subject | Neurosecretion | en |
dc.subject | Capacitance measurement | en |
dc.subject | Amperometry | en |
dc.title | Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2 | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.relation.publisherversion | http://dx.doi.org/10.1085/jgp.200308855 | |
dc.identifier.doi | 10.1085/jgp.200308855 | |
dc.journaltitle | The Journal of general physiology | es |
dc.publication.volumen | 122 | es |
dc.publication.issue | 3 | es |
dc.publication.initialPage | 265 | es |
dc.publication.endPage | 276 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/17816 |
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