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dc.creatorUreña López, Juan
dc.creatorFernández-Chacón, Rafael
dc.creatorRodríguez Benot, Alberto
dc.creatorÁlvarez de Toledo Naranjo, Guillermo
dc.creatorLópez Barneo, José
dc.date.accessioned2015-01-14T13:52:23Z
dc.date.available2015-01-14T13:52:23Z
dc.date.issued1994
dc.identifier.issn1091-6490es
dc.identifier.issn0027-8424es
dc.identifier.urihttp://hdl.handle.net/11441/17476
dc.description.abstractWe have investigated the changes of cytosolic [Ca2+1 and the secretory -activity in single glomus cells dispersed from rabbit carotid bodies during exposure to solutions with variable 02 tension (Po2). In normoxic conditions (Po2= 145 mmHg; 1 mmHg = 133 Pa), intracellular [Ca2+J was 58 1 29 nM, and switching to lo'iV P02 (between 10 and 60 mmHg) led to a reversible of [Ca2+1 up to 800 nM. The response to hypoxia completely disappeared after removal of external Ca2+ or with the addition of 0.2 mM Cd2+ to the external solution. These same solutions also abolished both the Ca2+ current of the cells and the increase of internal (Ca2+ elicited by hig external K+. Elevations of cytosolic [Ca2e+ in response to hypoxia or to direct membrane depolarization elicited the release of dopamine, which was detected by amperometric techniques. Dopamine secretion occurred in episodes of spike-like activity that appear to represent the release from single secretory vesicles. From the mean charge of well-resolved secretory events, we estimated the average number of dopamine molecules per vesicle to be =140,000, a value about 15 times smaller than a previous estimate in chromaffin granules of adrenomedullary cells. These results directly demonstrate in a single-cell preparation the secretory response of glomus cells to hypoxia. The data indicate that the enhancement of cellular excitability upon exposure to low Po2 results in Ca2+ entry through voltage-gated channels, which leads to an increase in intracellular [Ca2+J and exocytotic transmitter release.es
dc.language.isoenges
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America, 91 (21), 10208-10211.es
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleHypoxia induces voltage-dependent Ca2+ entry and quantal dopamine secretion in carotid body glomus cellses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiología Médica y Biofísicaes
dc.journaltitleProceedings of the National Academy of Sciences of the United States of Americaes
dc.publication.volumen91es
dc.publication.issue21es
dc.publication.initialPage10208es
dc.publication.endPage10211es
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/17476

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