dc.creator | Gascón, Sergio | es |
dc.creator | Páez Gómez, Juan A. | es |
dc.creator | Díaz-Guerra, Margarita | es |
dc.creator | Scheiffele, Peter | es |
dc.creator | Gómez Scholl, Francisco Manuel | es |
dc.date.accessioned | 2024-07-31T07:59:09Z | |
dc.date.available | 2024-07-31T07:59:09Z | |
dc.date.issued | 2008-02-15 | |
dc.identifier.citation | Gascón, S., Páez Gómez, J.A., Díaz-Guerra, M., Scheiffele, P. y Gómez Scholl, F.M. (2008). Dual-promoter lentiviral vectors for constitutive and regulated gene expression in neurons. Journal of Neuroscience Methods, 168 (1), 104-112. https://doi.org/10.1016/j.jneumeth.2007.09.023. | |
dc.identifier.issn | 0165-0270 | es |
dc.identifier.issn | 1872-678X | es |
dc.identifier.uri | https://hdl.handle.net/11441/161780 | |
dc.description.abstract | Gene transfer methods for efficient co-expression of exogenous proteins in neurons are crucial tools towards the understanding of the molecular basis of the central nervous system. Lentiviruses are retroviral vectors that can transduce a wide variety of cells including differentiated neurons. In this work, we have generated lentiviral vectors containing dual promoters that allow efficient co-expression of exogenous proteins in neurons. We show that insertion of two copies of a human synapsin promoter/WPRE cassette in a single lentiviral vector directs robust co-expression of cDNAs in cultured neurons, while excluding expression in the surrounding glial cells. Furthermore, insertion of the tetracycline-inducible system (Tet-off) controlled by the synapsin promoter results in tightly regulated expression of EGFP when used as a transgene in cultured neurons. Transduction of primary neurons with this inducible system leads to a 100-fold increase in EGFP mRNA levels in the absence of doxycycline. In transduced cultures, EGFP transcription is inhibited within 24 h upon addition of doxycycline. The viral systems we developed here provide neuron-specific and regulated expression mediated by single lentiviral vectors and will prove valuable tools for the study of neuronal function. | es |
dc.format | application/pdf | es |
dc.format.extent | 31 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Neuroscience Methods, 168 (1), 104-112. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | lentivirus | es |
dc.subject | synapsin promoter | es |
dc.subject | inducible expression | es |
dc.subject | primary neurons | es |
dc.title | Dual-promoter lentiviral vectors for constitutive and regulated gene expression in neurons | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.date.embargoEndDate | 2009-02-15 | |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/abs/pii/S0165027007004773?via%3Dihub | es |
dc.identifier.doi | 10.1016/j.jneumeth.2007.09.023 | es |
dc.journaltitle | Journal of Neuroscience Methods | es |
dc.publication.volumen | 168 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 104 | es |
dc.publication.endPage | 112 | es |