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dc.creatorGallardo Fernández, Martaes
dc.creatorGarcía, Ana Ritaes
dc.creatorHornedo Ortega, Ruthes
dc.creatorTroncoso González, Ana Maríaes
dc.creatorGarcía Parrilla, María del Carmenes
dc.creatorBrito, M. Alexandraes
dc.date.accessioned2024-06-05T15:07:49Z
dc.date.available2024-06-05T15:07:49Z
dc.date.issued2024
dc.identifier.citationGallardo Fernández, M., García, A.R., Hornedo Ortega, R., Troncoso González, A.M., García Parrilla, M.d.C. y Brito, M.A. (2024). In Vitro Study of the Blood-brain Barrier Transport of Bioactives from Mediterranean Foods. Food and Function, 15 (7), 3420-3432. https://doi.org/10.1039/d3fo04760a.
dc.identifier.issn2042-6496es
dc.identifier.issn2042-650Xes
dc.identifier.urihttps://hdl.handle.net/11441/159817
dc.description.abstractThe Mediterranean diet (MD), characterized by olive oil, olives, fruits, vegetables, and wine intake, is associated with a reduced risk of dementia. These foods are rich in bioactives with neuroprotective and antioxidant properties, including hydroxytyrosol (HT), tyrosol (TYRS), serotonin (SER) and protocatechuic acid (PCA), a phenolic acid metabolite of anthocyanins. It remains to be established if these molecules cross the blood-brain barrier (BBB), a complex interface that strictly controls the entrance of molecules into the brain. We aimed to assess the ability of tyrosine (TYR), HT, TYRS, PCA and SER to pass through the BBB without disrupting its properties. Using Human Brain Microvascular Endothelial Cells as an in vitro model of the BBB, we assessed its integrity by transendothelial electrical resistance, paracellular permeability and immunocytochemical assays of the adherens junction protein β-catenin. The transport across the BBB was evaluated by ultra-high-performance liquid chromatography high resolution mass spectrometry. Results show that tested bioactives did not impair BBB integrity regardless of the concentration evaluated. Additionally, all of them cross the BBB, with the following percentages: HT (∼70%), TYR (∼50%), TYRS (∼30%), SER (∼30%) and PCA (∼9%). These results provide a basis for the MD neuroprotective role.es
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades AGL 2016-77505-C3-2-Res
dc.description.sponsorshipMinisterio de Ciencia e Innovación PID2019-108722RB-C32, IJC2020-045695-Ies
dc.description.sponsorshipFundação para a Ciência e a Tecnologia UIDB/04138/2020, UIDP/04138/2020es
dc.formatapplication/pdfes
dc.format.extent3 p.es
dc.language.isoenges
dc.publisherRoyal Society of Chemistryes
dc.relation.ispartofFood and Function, 15 (7), 3420-3432.
dc.rightsAttribution-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/*
dc.titleIn Vitro Study of the Blood-brain Barrier Transport of Bioactives from Mediterranean Foodses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legales
dc.relation.projectIDPID2019-108722RB-C32es
dc.relation.projectIDAGL 2016-77505-C3-2-Res
dc.relation.projectIDIJC2020-045695-Ies
dc.relation.projectIDUIDB/04138/2020es
dc.relation.projectIDUIDP/04138/2020es
dc.relation.publisherversionhttps://dx.doi.org/10.1039/d3fo04760aes
dc.identifier.doi10.1039/d3fo04760aes
dc.journaltitleFood and Functiones
dc.publication.volumen15es
dc.publication.issue7es
dc.publication.initialPage3420es
dc.publication.endPage3432es
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). Españaes
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (MICINN). Españaes
dc.contributor.funderFundação para a Ciência e a Tecnologia. Portugales

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