dc.creator | Gallardo Fernández, Marta | es |
dc.creator | García, Ana Rita | es |
dc.creator | Hornedo Ortega, Ruth | es |
dc.creator | Troncoso González, Ana María | es |
dc.creator | García Parrilla, María del Carmen | es |
dc.creator | Brito, M. Alexandra | es |
dc.date.accessioned | 2024-06-05T15:07:49Z | |
dc.date.available | 2024-06-05T15:07:49Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Gallardo Fernández, M., García, A.R., Hornedo Ortega, R., Troncoso González, A.M., García Parrilla, M.d.C. y Brito, M.A. (2024). In Vitro Study of the Blood-brain Barrier Transport of Bioactives from Mediterranean Foods. Food and Function, 15 (7), 3420-3432. https://doi.org/10.1039/d3fo04760a. | |
dc.identifier.issn | 2042-6496 | es |
dc.identifier.issn | 2042-650X | es |
dc.identifier.uri | https://hdl.handle.net/11441/159817 | |
dc.description.abstract | The Mediterranean diet (MD), characterized by olive oil, olives, fruits, vegetables, and wine intake, is associated with a reduced risk of dementia. These foods are rich in bioactives with neuroprotective and antioxidant properties, including hydroxytyrosol (HT), tyrosol (TYRS), serotonin (SER) and protocatechuic acid (PCA), a phenolic acid metabolite of anthocyanins. It remains to be established if these molecules cross the blood-brain barrier (BBB), a complex interface that strictly controls the entrance of molecules into the brain. We aimed to assess the ability of tyrosine (TYR), HT, TYRS, PCA and SER to pass through the BBB without disrupting its properties. Using Human Brain Microvascular Endothelial Cells as an in vitro model of the BBB, we assessed its integrity by transendothelial electrical resistance, paracellular permeability and immunocytochemical assays of the adherens junction protein β-catenin. The transport across the BBB was evaluated by ultra-high-performance liquid chromatography high resolution mass spectrometry. Results show that tested bioactives did not impair BBB integrity regardless of the concentration evaluated. Additionally, all of them cross the BBB, with the following percentages: HT (∼70%), TYR (∼50%), TYRS (∼30%), SER (∼30%) and PCA (∼9%). These results provide a basis for the MD neuroprotective role. | es |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades AGL 2016-77505-C3-2-R | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2019-108722RB-C32, IJC2020-045695-I | es |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia UIDB/04138/2020, UIDP/04138/2020 | es |
dc.format | application/pdf | es |
dc.format.extent | 3 p. | es |
dc.language.iso | eng | es |
dc.publisher | Royal Society of Chemistry | es |
dc.relation.ispartof | Food and Function, 15 (7), 3420-3432. | |
dc.rights | Attribution-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/4.0/ | * |
dc.title | In Vitro Study of the Blood-brain Barrier Transport of Bioactives from Mediterranean Foods | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.relation.projectID | PID2019-108722RB-C32 | es |
dc.relation.projectID | AGL 2016-77505-C3-2-R | es |
dc.relation.projectID | IJC2020-045695-I | es |
dc.relation.projectID | UIDB/04138/2020 | es |
dc.relation.projectID | UIDP/04138/2020 | es |
dc.relation.publisherversion | https://dx.doi.org/10.1039/d3fo04760a | es |
dc.identifier.doi | 10.1039/d3fo04760a | es |
dc.journaltitle | Food and Function | es |
dc.publication.volumen | 15 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 3420 | es |
dc.publication.endPage | 3432 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (MICINN). España | es |
dc.contributor.funder | Fundação para a Ciência e a Tecnologia. Portugal | es |