dc.creator | Hinojosa, M. G. | es |
dc.creator | Johansson, Y. | es |
dc.creator | Jos Gallego, Ángeles Mencía | es |
dc.creator | Cameán Fernández, Ana María | es |
dc.creator | Forsby, A. | es |
dc.date.accessioned | 2024-06-05T13:48:35Z | |
dc.date.available | 2024-06-05T13:48:35Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Hinojosa, M.G., Johansson, Y., Jos Gallego, Á.M., Cameán Fernández, A.M. y Forsby, A. (2024). Effects of Cylindrospermopsin, Chlorpyrifos and their Combination in a SH-SY5Y Cell Model Concerning Developmental Neurotoxicity. Ecotoxicology and Environmental Safety, 269, 115804. https://doi.org/10.1016/j.ecoenv.2023.115804. | |
dc.identifier.issn | 0147-6513 | es |
dc.identifier.issn | 1090-2414 | es |
dc.identifier.uri | https://hdl.handle.net/11441/159810 | |
dc.description.abstract | The cyanotoxin cylindrospermopsin (CYN) has been postulated to cause neurotoxicity, although the studies in this concern are very few. In addition, some studies in vitro indicate its possible effects on development. Furthermore, pesticides can be present in the same environmental samples as cyanotoxins. Therefore, chlorpyrifos (CPF) has been one of the most common pesticides used worldwide. The aim of this report was to study the effects of CYN, isolated and in combination with CPF, in a developmental neurotoxicity in vitro model. The human neuroblastoma SH-SY5Y cell line was exposed during 6 days of differentiation to both toxics to study their effects on cell viability and neurite outgrowth. To further evaluate effects of both toxicants on cholinergic signaling, their agonistic and antagonistic activities on the α7 homomeric nicotinic acetylcholine receptor (nAChR) were studied upon acute exposure. Moreover, a transcriptomic analysis by qPCR was performed after 6 days of CYN-exposure during differentiation. The results showed a concentration-dependent decrease on both cell viability and neurite outgrowth for both toxics isolated, leading to effective concentration 20 (EC20) values of 0.35 µM and 0.097 µM for CYN on cell viability and neurite outgrowth, respectively, and 100 µM and 58 µM for CPF, while the combination demonstrated no significant variations. In addition, 95 µM and 285 µM CPF demonstrated to act as an antagonist to nicotine on the nAChR, although CYN up to 2.4 µM had no effect on the efficacy of these receptors. Additionally, the EC20 for CYN (0.097 µM) on neurite outgrowth downregulated expression of the 5 genes NTNG2 (netrin G2), KCNJ11 (potassium channel), SLC18A3 (vesicular acetylcholine transporter), APOE (apolipoprotein E), and SEMA6B (semaphorin 6B), that are all important for neuronal development. Thus, this study points out the importance of studying the effects of CYN in terms of neurotoxicity and developmental neurotoxicity. | es |
dc.description.sponsorship | Swedish Fund for Research Without Animal Experiments N2018-0004, F2019-0009, F2020-0006 | es |
dc.description.sponsorship | Swedish Research Council 2018-03269 | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad AGL2015-64558-R | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2019-104890RB-I00 | es |
dc.format | application/pdf | es |
dc.format.extent | 11 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Ecotoxicology and Environmental Safety, 269, 115804. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Chlorpyrifos | es |
dc.subject | Cylindrospermopsin | es |
dc.subject | Developmental neurotoxicity | es |
dc.subject | NAChRs | es |
dc.subject | Neurite outgrowth | es |
dc.subject | Transcriptomics | es |
dc.title | Effects of Cylindrospermopsin, Chlorpyrifos and their Combination in a SH-SY5Y Cell Model Concerning Developmental Neurotoxicity | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.relation.projectID | N2018-0004 | es |
dc.relation.projectID | F2019-0009 | es |
dc.relation.projectID | F2020-0006 | es |
dc.relation.projectID | 2018-03269 | es |
dc.relation.projectID | AGL2015-64558-R | es |
dc.relation.projectID | PID2019-104890RB-I00 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.ecoenv.2023.115804 | es |
dc.identifier.doi | 10.1016/j.ecoenv.2023.115804 | es |
dc.journaltitle | Ecotoxicology and Environmental Safety | es |
dc.publication.volumen | 269 | es |
dc.publication.initialPage | 115804 | es |
dc.contributor.funder | Swedish Fund for Research Without Animal Experiments | es |
dc.contributor.funder | Swedish Research Council | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |