dc.creator | Ortiz Cerda, Tamara Andrea | es |
dc.creator | Argüelles Arias, Federico | es |
dc.creator | Macías García, Laura | es |
dc.creator | Vázquez Román, María Victoria | es |
dc.creator | Tapia, Gladys | es |
dc.creator | Xie, Kangzhe | es |
dc.creator | García-García, María Desirée | es |
dc.creator | Merinero de los Santos, Manuel | es |
dc.creator | García Montes, Josefa María | es |
dc.creator | Alcudia Cruz, Ana | es |
dc.creator | Witting, Paul K. | es |
dc.creator | Miguel Rodríguez, Manuel de | es |
dc.date.accessioned | 2024-05-29T12:27:27Z | |
dc.date.available | 2024-05-29T12:27:27Z | |
dc.date.issued | 2024-01-12 | |
dc.identifier.citation | Ortiz Cerda, T.A., Argüelles Arias, F., Macías García, L., Vázquez Román, M.V., Tapia, G., Xie, K.,...,Miguel Rodríguez, M.d. (2024). Effects of polyphenolic maqui ( Aristotelia chilensis ) extract on the inhibition of NLRP3 inflammasome and activation of mast cells in a mouse model of Crohn's disease-like colitis. Frontiers In Immunology, 14, 1229767. https://doi.org/10.3389/fimmu.2023.1229767. | |
dc.identifier.issn | 1664-3224 | es |
dc.identifier.uri | https://hdl.handle.net/11441/159443 | |
dc.description.abstract | Introduction: Crohn’s disease (CD) involves activation of mast cells (MC) and NFкB in parallel with the PPAR-a/NLRP3 inflammasome/IL-1b pathway in the
inflamed colon. Whether polyphenols from maqui (Aristotelia chilensis)
represent a natural alternative treatment for CD is unclear. Therefore, we used
an animal model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD-like
colitis to investigate protective effects of maqui extract through monitoring
NLRP3 inflammasome and MC activation in colon tissue.
Methods: Maqui extract was administered via orogastric route to mice after
(post-Treatment group) or prior (pre-Treatment group) to TNBS-induction.
Colon pathology was characterized by histoarchitectural imaging, disease
activity index (DAI), and assessing NF-кB, p-NF-кB, PPAR-a/NLRP3 expression
and IL-1b levels.
Results: Compared to mice treated with TNBS alone administration of
anthocyanin-rich maqui extract improved the DAI, colon histoarchitecture and
reduced both colon wet-weight and transmural inflammation. Induction with
TNBS significantly increased colonic NLPR3 inflammasome activation, while cotreatment with maqui extract (either post- or pre-Treatment) significantly
downregulated NLRP3, ASC and caspase-1 levels, which manifested as
reduced colonic IL-1b levels. Supplemented maqui extract marginally
diminished NF-кB activity in epithelial cells but reached statistical significance
in immune cells (as judged by decreased NF-кB phosphorylation). PPAR-a signaling was largely unaffected by Maqui whereas MC infiltration into the colon
mucosa and submucosa decreased and their level of degranulation
was suppressed.
Conclusion: These outcomes show the post- and pre- Treatment effect of a
polyphenolic extract rich in anthocyanins from maqui the acute phase of TNBSinduced CD-like colitis is linked to suppression of the NLRP3 inflammasome and
reduced MC responses. These data indicate that maqui extract represents a
potential nutraceutical for the treatment of inflammatory bowel disease (IBD). | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media S.A. | es |
dc.relation.ispartof | Frontiers In Immunology, 14, 1229767. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Crohn’s disease | es |
dc.subject | Polyphenols | es |
dc.subject | Anthocyanins | es |
dc.subject | Maqui | es |
dc.subject | Mast cells | es |
dc.subject | NLRP3 inflammasome | es |
dc.subject | Interleukin 1b | es |
dc.title | Effects of polyphenolic maqui ( Aristotelia chilensis ) extract on the inhibition of NLRP3 inflammasome and activation of mast cells in a mouse model of Crohn's disease-like colitis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | PAIDI CTS949 | es |
dc.relation.publisherversion | https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1229767/full | es |
dc.identifier.doi | 10.3389/fimmu.2023.1229767 | es |
dc.journaltitle | Frontiers In Immunology | es |
dc.publication.volumen | 14 | es |
dc.publication.initialPage | 1229767 | es |
dc.contributor.funder | Junta de Andalucía | es |