dc.creator | Balsera-Manzanero, María | es |
dc.creator | Ghirga, Francesca | es |
dc.creator | Ruiz-Molina, Ana | es |
dc.creator | Mori, Mattia | es |
dc.creator | Pachón Díaz, Jerónimo | es |
dc.creator | Botta, Bruno | es |
dc.creator | Cordero Matia, María Elisa | es |
dc.creator | Quaglio, Deborah | es |
dc.creator | Sanchez Cespedes, Javier | es |
dc.date.accessioned | 2024-03-11T14:57:38Z | |
dc.date.available | 2024-03-11T14:57:38Z | |
dc.date.issued | 2024-01-24 | |
dc.identifier.citation | Balsera-Manzanero, M., Ghirga, F., Ruiz-Molina, A., Mori, M., Pachón Díaz, J., Botta, B.,...,Sanchez Cespedes, J. (2024). Inhibition of adenovirus transport from the endosome to the cell nucleus by rotenone. Frontiers in Pharmacology, 14, 1293296. https://doi.org/10.3389/fphar.2023.1293296. | |
dc.identifier.issn | 1663-9812 | es |
dc.identifier.uri | https://hdl.handle.net/11441/156104 | |
dc.description.abstract | Regardless of the clinical impact of human adenovirus (HAdV) infections in the
healthy population and its high morbidity in immunosuppressed patients, a
specific treatment is still not yet available. In this study, we screened the
CM1407 COST Action’s chemical library, comprising 1,233 natural products to
identify compounds that restrict HAdV infection. Among them, we identified
rotenolone, a compound that significantly inhibited HAdV infection. Next, we
selected four isoflavonoid-type compounds (e.g., rotenone, deguelin, millettone,
and tephrosin), namely rotenoids, structurally related to rotenolone in order to
evaluate and characterized in vitro their antiviral activities against HAdV and
human cytomegalovirus (HCMV). Their IC50 values for HAdV ranged from
0.0039 µM for rotenone to 0.07 µM for tephrosin, with selective indices
ranging from 164.1 for rotenone to 2,429.3 for deguelin. In addition, the
inhibition of HCMV replication ranged from 50% to 92.1% at twice the IC50
concentrations obtained in the plaque assay for each compound against HAdV.
Our results indicated that the mechanisms of action of rotenolone, deguelin, and
tephrosin involve the late stages of the HAdV replication cycle. However, the
antiviral mechanism of action of rotenone appears to involve the alteration of the
microtubular polymerization, which prevents HAdV particles from reaching the
nuclear membrane of the cell. These isoflavonoid-type compounds exert high
antiviral activity against HAdV at nanomolar concentrations, and can be
considered strong hit candidates for the development of a new class of
broad-spectrum antiviral drugs. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 p. | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media S.A. | es |
dc.relation.ispartof | Frontiers in Pharmacology, 14, 1293296. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Rotenoids | es |
dc.subject | Adenovirus infection | es |
dc.subject | Antiviral compound | es |
dc.subject | Cytomegalovirus | es |
dc.subject | Microtubular polymerization | es |
dc.title | Inhibition of adenovirus transport from the endosome to the cell nucleus by rotenone | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.contributor.affiliation | Instituto de Biomedicina de Sevilla (IBIS) | es |
dc.relation.publisherversion | https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1293296/full | es |
dc.identifier.doi | 10.3389/fphar.2023.1293296 | es |
dc.journaltitle | Frontiers in Pharmacology | es |
dc.publication.volumen | 14 | es |
dc.publication.initialPage | 1293296 | es |