dc.creator | Bullones Bolaños, Andrea | es |
dc.creator | Martín Muñoz, Paula | es |
dc.creator | Vallejo Grijalba, Claudia | es |
dc.creator | Bernal Bayard, Joaquín | es |
dc.creator | Ramos Morales, Francisco | es |
dc.date.accessioned | 2024-02-12T14:32:26Z | |
dc.date.available | 2024-02-12T14:32:26Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Bullones Bolaños, A., Martín Muñoz, P., Vallejo Grijalba, C., Bernal Bayard, J. y Ramos Morales, F. (2024). Specificities and Redundancies in the NEL Family of Bacterial E3 Ubiquitin Ligases of Salmonella Enterica Serovar Typhimurium. Frontiers in Immunology, 15, 1328707. https://doi.org/10.3389/fimmu.2024.1328707. | |
dc.identifier.issn | 1664-3224 | es |
dc.identifier.uri | https://hdl.handle.net/11441/155167 | |
dc.description.abstract | Salmonella enterica serovar Typhimurium expresses two type III secretion
systems, T3SS1 and T3SS2, which are encoded in Salmonella pathogenicity
island 1 (SPI1) and SPI2, respectively. These are essential virulent factors that
secrete more than 40 effectors that are translocated into host animal cells. This
study focuses on three of these effectors, SlrP, SspH1, and SspH2, which are
members of the NEL family of E3 ubiquitin ligases. We compared their
expression, regulation, and translocation patterns, their role in cell invasion and
intracellular proliferation, their ability to interact and ubiquitinate specific host
partners, and their effect on cytokine secretion. We found that transcription of
the three genes encoding these effectors depends on the virulence regulator
PhoP. Although the three effectors have the potential to be secreted through
T3SS1 and T3SS2, the secretion of SspH1 and SspH2 is largely restricted to T3SS2
due to their expression pattern. We detected a role for these effectors in
proliferation inside fibroblasts that is masked by redundancy. The generation of
chimeric proteins allowed us to demonstrate that the N-terminal part of these
proteins, containing the leucine-rich repeat motifs, confers specificity towards
ubiquitination targets. Furthermore, the polyubiquitination patterns generated
were different for each effector, with Lys48 linkages being predominant for
SspH1 and SspH2. Finally, our experiments support an anti-inflammatory role for
SspH1 and SspH2. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2019-106132RB-I00 | es |
dc.description.sponsorship | Fondo Europeo de Desarrollo Regional P20_00576 | es |
dc.description.sponsorship | Universidad de Sevilla US-1380805 | es |
dc.format | application/pdf | es |
dc.format.extent | 20 p. | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media | es |
dc.relation.ispartof | Frontiers in Immunology, 15, 1328707. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Salmonella enterica | es |
dc.subject | Type III secretion systems | es |
dc.subject | E3 ubiquitin ligase | es |
dc.subject | SlrP | es |
dc.subject | SspH1 | es |
dc.subject | SspH2 | es |
dc.title | Specificities and Redundancies in the NEL Family of Bacterial E3 Ubiquitin Ligases of Salmonella Enterica Serovar Typhimurium | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | PID2019-106132RB-I00 | es |
dc.relation.projectID | P20_00576 | es |
dc.relation.projectID | US-1380805 | es |
dc.relation.publisherversion | https://doi.org/10.3389/fimmu.2024.1328707 | es |
dc.identifier.doi | 10.3389/fimmu.2024.1328707 | es |
dc.journaltitle | Frontiers in Immunology | es |
dc.publication.volumen | 15 | es |
dc.publication.initialPage | 1328707 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |
dc.contributor.funder | Universidad de Sevilla | es |