Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii colonization

Abstract

Real-time polymerase chain reaction (PCR)-based approaches have not been assessed in terms of their ability to detect patients colonized by Acinetobacter baumannii during active surveillance. This prospective, double-blind study demonstrated that a real-time PCR assay had high sensitivity (100%) and specificity (91.2%) compared with conven-tional culture for detecting A. baumannii in 397 active surveillance samples, and provided results within 3 h. Receiver-operator curve analyses demonstrated that the technique has diagnostic accuracy of 97.7% (95% confidence interval 96.0e99.3%). This method could facilitate the rapid implementation of infection control measures for preventing the transmission of A. baumannii.