Artículo
Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii colonization
Autor/es | Blanco Lobo, P.
González-Galán, V. García-Quintanilla, M. Valencia, R. Cazalla, A. Martín, C. Alonso, I. Pérez-Romero, P. Cisneros, José Miguel McConnell, M. J. |
Departamento | Universidad de Sevilla. Departamento de Medicina Preventiva y Salud Pública Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2016 |
Fecha de depósito | 2024-01-22 |
Publicado en |
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Resumen | Real-time polymerase chain reaction (PCR)-based approaches have not been assessed in terms of their ability to detect patients colonized by Acinetobacter baumannii during active surveillance. This prospective, double-blind ... Real-time polymerase chain reaction (PCR)-based approaches have not been assessed in terms of their ability to detect patients colonized by Acinetobacter baumannii during active surveillance. This prospective, double-blind study demonstrated that a real-time PCR assay had high sensitivity (100%) and specificity (91.2%) compared with conven-tional culture for detecting A. baumannii in 397 active surveillance samples, and provided results within 3 h. Receiver-operator curve analyses demonstrated that the technique has diagnostic accuracy of 97.7% (95% confidence interval 96.0e99.3%). This method could facilitate the rapid implementation of infection control measures for preventing the transmission of A. baumannii. |
Cita | Blanco Lobo, P., González-Galán, V., García-Quintanilla, M., Valencia, R., Cazalla, A., Martín, C.,...,McConnell, M.J. (2016). Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii colonization. JOURNAL OF HOSPITAL INFECTION, 94 (1), 68-71. https://doi.org/10.1016/j.jhin.2016.04.008. |
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