Artículo
BIChromET: A Chromogenic Culture Medium for Detection of Piperacillin/Tazobactam and Cefepime Resistance in Pseudomonas aeruginosa
Autor/es | Ortiz de la Rosa, José Manuel
Rodríguez Villodres, Ángel Martín Gutiérrez, Guillermo Cintora Mairal, Carmen García Escobar, José Luis Gálvez Benítez, Lydia Cisneros, José Miguel Lepe Jiménez, José Antonio |
Departamento | Universidad de Sevilla. Departamento de Medicina Universidad de Sevilla. Departamento de Microbiología |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-12-04 |
Publicado en |
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Resumen | Objectives: The BIChromET selective medium for detecting piperacillin-tazobactam (TZP) and cefepime (FEP) resistant Pseudomonas aeruginosa was developed. Methods: The performance of this medium was first evaluated using a ... Objectives: The BIChromET selective medium for detecting piperacillin-tazobactam (TZP) and cefepime (FEP) resistant Pseudomonas aeruginosa was developed. Methods: The performance of this medium was first evaluated using a collection of 100 P. aeruginosa clinical strains (70 TZP-susceptible, 30 TZP-resistant, 58 FEP-susceptible, and 42 FEP-resistant). Then, we performed clinical validation by testing 173 respiratory clinical samples. Results: The BIChromET medium showed excellent sensitivity (TZP (avg. 96.7%); FEP (avg. 92.7%)) and specificity (TZP (avg. 98.9%); FEP (avg. 98%)) in distinguishing the detection limit ranging from 104 to 108 CFU/mL. Then, testing the bronchoalveolar lavage (BAL) and tracheobronchial aspirate (TBA) clinical specimens (N = 173) revealed the excellent performance of the medium with P. aeruginosa, showing 100% and 92.6% of categorical agreements with the results obtained via the broth microdilution methods (BMD) for TZP and FEP, respectively. Conclusion: This medium allows for easy and accurate detection of TZP/FEP-resistant isolates regardless of their resistance mechanisms. |
Cita | Ortiz de la Rosa, J.M., Rodríguez Villodres, Á., Martín Gutiérrez, G., Cintora Mairal, C., García Escobar, J.L., Gálvez Benítez, L.,...,Lepe Jiménez, J.A. (2023). BIChromET: A Chromogenic Culture Medium for Detection of Piperacillin/Tazobactam and Cefepime Resistance in Pseudomonas aeruginosa. Antibiotics, 12 (11), 1573. https://doi.org/10.3390/antibiotics12111573. |
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