dc.creator | Ortiz de la Rosa, José Manuel | es |
dc.creator | Rodríguez Villodres, Ángel | es |
dc.creator | Martín Gutiérrez, Guillermo | es |
dc.creator | Cintora Mairal, Carmen | es |
dc.creator | García Escobar, José Luis | es |
dc.creator | Gálvez Benítez, Lydia | es |
dc.creator | Cisneros, José Miguel | es |
dc.creator | Lepe Jiménez, José Antonio | es |
dc.date.accessioned | 2023-12-04T08:45:10Z | |
dc.date.available | 2023-12-04T08:45:10Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Ortiz de la Rosa, J.M., Rodríguez Villodres, Á., Martín Gutiérrez, G., Cintora Mairal, C., García Escobar, J.L., Gálvez Benítez, L.,...,Lepe Jiménez, J.A. (2023). BIChromET: A Chromogenic Culture Medium for Detection of Piperacillin/Tazobactam and Cefepime Resistance in Pseudomonas aeruginosa. Antibiotics, 12 (11), 1573. https://doi.org/10.3390/antibiotics12111573. | |
dc.identifier.issn | 2079-6382 | es |
dc.identifier.uri | https://hdl.handle.net/11441/152106 | |
dc.description.abstract | Objectives: The BIChromET selective medium for detecting piperacillin-tazobactam (TZP) and cefepime (FEP) resistant Pseudomonas aeruginosa was developed. Methods: The performance of this medium was first evaluated using a collection of 100 P. aeruginosa clinical strains (70 TZP-susceptible, 30 TZP-resistant, 58 FEP-susceptible, and 42 FEP-resistant). Then, we performed clinical validation by testing 173 respiratory clinical samples. Results: The BIChromET medium showed excellent sensitivity (TZP (avg. 96.7%); FEP (avg. 92.7%)) and specificity (TZP (avg. 98.9%); FEP (avg. 98%)) in distinguishing the detection limit ranging from 104 to 108 CFU/mL. Then, testing the bronchoalveolar lavage (BAL) and tracheobronchial aspirate (TBA) clinical specimens (N = 173) revealed the excellent performance of the medium with P. aeruginosa, showing 100% and 92.6% of categorical agreements with the results obtained via the broth microdilution methods (BMD) for TZP and FEP, respectively. Conclusion: This medium allows for easy and accurate detection of TZP/FEP-resistant isolates regardless of their resistance mechanisms. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Antibiotics, 12 (11), 1573. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | piperacillin/tazobactam | es |
dc.subject | Cefepime | es |
dc.subject | Empiric treatment | es |
dc.subject | Pseudomonas aeruginosa | es |
dc.title | BIChromET: A Chromogenic Culture Medium for Detection of Piperacillin/Tazobactam and Cefepime Resistance in Pseudomonas aeruginosa | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | https://www.mdpi.com/2079-6382/12/11/1573 | es |
dc.identifier.doi | 10.3390/antibiotics12111573 | es |
dc.contributor.group | Universidad de Sevilla. CTS 203: Estudio de las Enfermedades Infecciosas | es |
dc.journaltitle | Antibiotics | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 11 | es |
dc.publication.initialPage | 1573 | es |