dc.creator | Adanma Obara, Elisabeth Anne | es |
dc.creator | Aguilar Morante, Diana | es |
dc.creator | Darling Rasmussen, Rikke | es |
dc.creator | Frias, Alex | es |
dc.creator | Vitting-Serup, Kristoffer | es |
dc.creator | Chieh Lim, Yi | es |
dc.creator | Hamerlik, Petra | es |
dc.date.accessioned | 2023-11-09T09:55:10Z | |
dc.date.available | 2023-11-09T09:55:10Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Adanma Obara, E.A., Aguilar Morante, D., Darling Rasmussen, R., Frias, A., Vitting-Serup, K., Chieh Lim, Y. y Hamerlik, P. (2020). SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells. Nature Communications, 11 (1), 1-14. https://doi.org/10.1038/s41467-020-18549-8. | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | https://hdl.handle.net/11441/150366 | |
dc.description.abstract | Glioblastoma cancer-stem like cells (GSCs) display marked resistance to ionizing radiation (IR), a standard of care for glioblastoma patients. Mechanisms underpinning radio-resistance of GSCs remain largely unknown. Chromatin state and the accessibility of DNA lesions to DNA repair machineries are crucial for the maintenance of genomic stability. Understanding the functional impact of chromatin remodeling on DNA repair in GSCs may lay the foundation for advancing the efficacy of radio-sensitizing therapies. Here, we present the results of a high-content siRNA microscopy screen, revealing the transcriptional elongation factor SPT6 to be critical for the genomic stability and self-renewal of GSCs. Mechanistically, SPT6 transcriptionally up-regulates BRCA1 and thereby drives an error-free DNA repair in GSCs. SPT6 loss impairs the self-renewal, genomic stability and tumor initiating capacity of GSCs. Collectively, our results provide mechanistic insights into how SPT6 regulates DNA repair and identify SPT6 as a putative therapeutic target in glioblastoma. | es |
dc.description.sponsorship | Danish Cancer Society R146-A9511 | es |
dc.description.sponsorship | Danish Cancer Society R148-A10151 | es |
dc.description.sponsorship | Danish Council for Independent Research | es |
dc.description.sponsorship | Danish National Research Foundation (CARD) | es |
dc.description.sponsorship | Lundbeck Foundation | es |
dc.description.sponsorship | Novo Nordisk Foundation NNF17OC0026056 | es |
dc.description.sponsorship | Novo Nordisk Foundation NNF16OC0023146 | es |
dc.description.sponsorship | Swedish research council | es |
dc.format | application/pdf | es |
dc.format.extent | 14 | es |
dc.language.iso | eng | es |
dc.publisher | Nature Portfolio | es |
dc.relation.ispartof | Nature Communications, 11 (1), 1-14. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Glioblastoma cancer-stem like cells (GSCs) | es |
dc.subject | Ionizing radiation (IR) | es |
dc.subject | Radio-resistance | es |
dc.subject | DNA | es |
dc.subject | SPT6 | es |
dc.title | SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.identifier.doi | 10.1038/s41467-020-18549-8 | es |
dc.contributor.group | Universidad de Sevilla. CTS-007: Fisiopatología de Células Madre Neurales | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 11 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 14 | es |