dc.creator | Franco Losilla, Marta | es |
dc.creator | Nordzieke, Steffen | es |
dc.creator | Feldmann, Ingo | es |
dc.creator | Limón Mirón, María del Carmen | es |
dc.creator | Ávalos Cordero, Francisco Javier | es |
dc.date.accessioned | 2023-09-12T14:03:37Z | |
dc.date.available | 2023-09-12T14:03:37Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Franco Losilla, M., Nordzieke, S., Feldmann, I., Limón Mirón, M.d.C. y Ávalos Cordero, F.J. (2023). HmbC, a Protein of the HMG Family, Participates in the Regulation of Carotenoid Biosynthesis in Fusarium fujikuroi. Genes, 14 (8), 1661. https://doi.org/10.3390/genes14081661. | |
dc.identifier.issn | 2073-4425 | es |
dc.identifier.uri | https://hdl.handle.net/11441/148882 | |
dc.description.abstract | In the fungus Fusarium fujikuroi, carotenoid production is up-regulated by light and down-regulated by the CarS RING finger protein, which modulates the mRNA levels of carotenoid pathway genes (car genes). To identify new potential regulators of car genes, we used a biotin-mediated pull-down procedure to detect proteins capable of binding to their promoters. We focused our attention on one of the proteins found in the screening, belonging to the High-Mobility Group (HMG) family that was named HmbC. The deletion of the hmbC gene resulted in increased carotenoid production due to higher mRNA levels of car biosynthetic genes. In addition, the deletion resulted in reduced carS mRNA levels, which could also explain the partial deregulation of the carotenoid pathway. The mutants exhibited other phenotypic traits, such as alterations in development under certain stress conditions, or reduced sensitivity to cell wall degrading enzymes, revealed by less efficient protoplast formation, indicating that HmbC is also involved in other cellular processes. In conclusion, we identified a protein of the HMG family that participates in the regulation of carotenoid biosynthesis. This is probably achieved through an epigenetic mechanism related to chromatin structure, as is frequent in this class of proteins. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BIO 2015–69613-R | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación RTI 2018-101902-B-I00 | es |
dc.description.sponsorship | Junta de Andalucía P10-CTS-6638, P20-01243 | es |
dc.format | application/pdf | es |
dc.format.extent | 18 p. | es |
dc.language.iso | eng | es |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es |
dc.relation.ispartof | Genes, 14 (8), 1661. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Carotenoids | es |
dc.subject | CarS gene | es |
dc.subject | High-mobility group | es |
dc.subject | HmbC protein | es |
dc.subject | Pprotoplast formation | es |
dc.subject | Pull-down assay | es |
dc.title | HmbC, a Protein of the HMG Family, Participates in the Regulation of Carotenoid Biosynthesis in Fusarium fujikuroi | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BIO 2015–69613-R | es |
dc.relation.projectID | RTI 2018-101902-B-I00 | es |
dc.relation.projectID | P10-CTS-6638 | es |
dc.relation.projectID | P20-01243 | es |
dc.relation.publisherversion | https://doi.org/10.3390/genes14081661 | es |
dc.identifier.doi | 10.3390/genes14081661 | es |
dc.journaltitle | Genes | es |
dc.publication.volumen | 14 | es |
dc.publication.issue | 8 | es |
dc.publication.initialPage | 1661 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Junta de Andalucía | es |